Cell type-specific involvement of RIG-I in antiviral response
Cell type-specific involvement of RIG-I in antiviral response
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DOI：
10.1016/j.immuni.2005.04.010
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发表时间：
2005-07-01
期刊：
影响因子：
32.4
通讯作者：
Akira, S
中科院分区：
文献类型：
--
作者：
Kato, H;Sato, S;Akira, S
Toll样受体通过识别病毒成分，在抗病毒反应中发挥重要作用。最近，一种RNA解旋酶RIG-I也被建议用于识别病毒双链RNA。然而，这些分子如何在体内对病毒识别做出贡献还知之甚少。我们通过基因打靶表明，RIG-I在感染RNA病毒后在成纤维细胞和常规树突状细胞(DC)中诱导I型干扰素(IFN)是必不可少的。Rig-I通过I-kappa B蛋白相关的激酶激活IRF3，从而诱导I型IFN。相比之下，产生大量干扰素-α的浆细胞样树突状细胞使用TLR系统而不是RIG-I进行病毒检测。总而言之，RIG-I和TLR系统以特定细胞类型的方式发挥抗病毒反应。
Toll-like receptors (TLRs) play an important role in antiviral response by recognizing viral components. Recently, a RNA helicase, RIG-I, was also suggested to recognize viral double-stranded RNA. However, how these molecules contribute to viral recognition in vivo is poorly understood. We show by gene targeting that RIG-I is essential for induction of type I interferons (IFNs) after infection with RNA viruses in fibroblasts and conventional dendritic cells (DCs). RIG-I induces type I IFNs by activating IRF3 via I kappa B kinase-related kinases. In contrast, plasmacytoid DCs, which produce large amounts of IFN-alpha, use the TLR system rather than RIG-I for viral detection. Taken together, RIG-I and the TLR system exert antiviral responses in a cell type-specific manner.