Modification of agarose with carboxylation and grafting dopamine for promotion of its cell-adhesiveness
Modification of agarose with carboxylation and grafting dopamine for promotion of its cell-adhesiveness
复制标题
羧化修饰琼脂糖并接枝多巴胺以提高其细胞粘附性
DOI：
10.1016/j.carbpol.2012.12.003
复制
发表时间：
2013-02-15
影响因子：
11.2
通讯作者：
Tang, Shunqing
中科院分区：
文献类型：
--
作者：
Su, Yixue;Chu, Bin;Tang, Shunqing
为了提高琼脂糖的生物活性，我们采用羧化和接枝多巴胺对琼脂糖进行修饰。在碱性条件下，采用2,2,6,6-四甲基哌啶-1-氧自由基（TEMPO）氧化体系，将d -半乳糖环上的C-6羟基氧化为羧基，制备羧基化琼脂糖，羧基化程度最高可达30%。通过凝胶渗透色谱（GPC）测定，随着氧化剂用量的增加，羧化琼脂糖的分子量降至4 kDa。羧化琼脂糖与多巴胺通过EDC缩合反应反应得到琼脂糖接枝多巴胺（Ag-g-DA）， 280 nm紫外光谱测得多巴胺接枝率为9.3%。通过红外光谱（FT-IR）和核磁共振（C-13 NMR）对改性琼脂糖的结构进行了表征。羧化琼脂糖和Ag-g-DA均无细胞毒性，并能促进细胞粘附。(C) 2012 Elsevier Ltd.版权所有。
In order to improve bioactivity of agarose, we modified agarose by carboxylation and grafting dopamine. Under alkaline condition, carboxylated agarose was prepared using 2,2,6,6-tetramethylpiperidine-1-oxyl radical (TEMPO) oxidation system by oxidizing C-6 hydroxyl on D-galactose ring into carboxyl group, and the maximum value of the degree of carboxylation reached 30%. With the increase of the amount of oxidant, the molecular weight of the carboxylated agarose decreased to 4 kDa by gel permeation chromatography (GPC) measure. Carboxylated agarose reacted with dopamine through EDC condensation reaction to obtain agarose grafting dopamine (Ag-g-DA), and the grafting rate of dopamine was determined to be 9.3% by UV spectroscopy at 280 nm. The structures of these modified agaroses were determined by FT-IR and C-13 NMR. Both carboxylated agarose and Ag-g-DA showed no cytotoxicity and promoted cell-adhesiveness. (C) 2012 Elsevier Ltd. All rights reserved.