AN INTRON-ENCODED PROTEIN IS ACTIVE IN A GENE CONVERSION PROCESS THAT SPREADS AN INTRON INTO A MITOCHONDRIAL GENE
AN INTRON-ENCODED PROTEIN IS ACTIVE IN A GENE CONVERSION PROCESS THAT SPREADS AN INTRON INTO A MITOCHONDRIAL GENE
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DOI：
10.1016/s0092-8674(85)80011-8
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发表时间：
1985-01-01
期刊：
影响因子：
64.5
通讯作者：
DUJON, B
中科院分区：
文献类型：
--
作者：
JACQUIER, A;DUJON, B
酿酒酵母（Saccharomycescerevisiae）线粒体21 S rRNA基因的内含子具有在各种酵母物种中保守的长的内部阅读框（ORF）。在正内含子和负内含子变体之间的杂交中，该内含子决定了特定的基因转换现象，这导致内含子序列整合到基因的所有先前的负内含子拷贝中。从内含子内的移码突变体，该ORF和线粒体蛋白质合成的需要表明ORF编码在基因转换中具有活性的蛋白质，该基因转换在杂交菌株的群体内传播内含子。这种新的内含子功能让人想起“转座酶”编码的移动的遗传元件，并讨论了与其他内含子功能。
The intron of the mitochondrial 21S rRNA gene of Saccharomyces cerevisiae possesses a long internal reading frame (ORF) that is conserved in various yeast species. In crosses between intron-plus and intron-minus variants, this intron determines a specific gene conversion phenomenon, which results in the integration of the intron sequence within all previously intron-minus copies of the gene. From a frameshift mutant within the intron this ORF and the need for mitochrondrial protein synthesis shows that ORF encodes a protein active in the gene conversion that spreads the intron within populations of interbreeding strains. This new intron function is reminiscent of the "transposase" encoded by mobile genetic elements and is discussed in relation to other intron functions.