Binding of peanut lectin to germinal-centre cells: a marker for B-cell subsets of follicular lymphoma?
Binding of peanut lectin to germinal-centre cells: a marker for B-cell subsets of follicular lymphoma?
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DOI：
10.1038/bjc.1981.149
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发表时间：
1981-07
影响因子：
8.8
通讯作者：
Davies, A J
中科院分区：
文献类型：
--
作者：
Rose, M L;Habeshaw, J A;Kennedy, R;Sloane, J;Wiltshaw, E;Davies, A J
辣根过氧化物酶标记的花生凝集素（HRP-PNL）的扁桃体，淋巴瘤淋巴结，反应性淋巴结和杂项肿瘤的冷冻切片的结合表明，PNL结合选择性淋巴细胞在生发中心。将21例非霍奇金淋巴瘤患者的淋巴结作为细胞悬液进行表型分析，以检测PNL结合和以下表面标志物：E玫瑰花结、C3 d、SIG、T细胞亚群的OK标志物、IG重链和轻链类别。PNL结合率与具有Sig和C3 d受体的细胞呈正相关。4/5例中心母细胞/中心细胞滤泡性淋巴瘤具有PNL+ SIG+ C3 d+表型。2例中心母细胞/中心细胞弥漫性淋巴瘤均为PNL-。PNL结合与重链或轻链IG类别之间无相关性。PNL结合和C3 d受体的存在并不总是正相关的，表明滤泡细胞可能是PNL+ SIg+ C3 d+或PNL+ SIg+ C3 d-。PNL与1例胸腺增生和2例T型淋巴母细胞性恶性淋巴瘤的结合模式提示部分胸腺皮质细胞与PNL结合。
The binding of horseradish-peroxidase-labelled peanut lectin (HRP-PNL) to cryostat sections of tonsil, lymphoma lymph nodes, reactive lymph nodes and miscellaneous tumours demonstrated that PNL binds selectively to lymphocytes in germinal centres. Lymph nodes from 21 patients with non-Hodgkin's lymphomas were phenotyped as cell suspensions for PNL binding, and the following surface markers: E rosetting, C3d, SIg, OK markers of T-cell subsets, Ig heavy-chain and light-chain classes. There was a positive correlation between PNL binding and cells with SIg and C3d receptors. 4/5 cases of centroblastic/centrocytic follicular lymphoma had a PNL+ SIg+ C3d+ phenotype. Both cases of centroblastic/centrocytic diffuse lymphoma were PNL-. There was no correlation between PNL binding and heavy- or light-chain Ig class. PNL binding and presence of C3d receptors were not always positively correlated, indicating that follicular cells may be either PNL+ SIg+ C3d+ or PNL+ SIg+ C3d-. The binding pattern of PNL to 1 case of thymic hyperplasia and 2 cases of malignant lymphoma lymphoblastic T type suggested that some but not all cortical thymocytes bind PNL.