Achromosomal cleavage of fertilized starfish eggs in the presence of aphidicolin.
Achromosomal cleavage of fertilized starfish eggs in the presence of aphidicolin.
复制标题
在阿菲迪霉素存在下，受精海星卵的无染色体分裂。
DOI：
10.1016/0012-1606(81)90272-4
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发表时间：
1981
影响因子：
2.7
通讯作者：
S. Ikegami
中科院分区：
文献类型：
--
作者：
H. Nagano;S. Hirai;K. Okano;S. Ikegami
研究了真核生物DNA合成特异性抑制剂阿菲霉素对海星（Asterina pectinifera）受精卵发育的影响。受精卵在阿霉素存在的情况下继续分裂8或9次，尽管观察到发育的延迟和卵裂球的大小不相等。在阿霉素存在下发育的胚胎的卵裂球中，细胞核和染色体未被检测到，尽管在细胞质中可以清楚地看到圆形细胞。这些胚胎的DNA合成几乎完全被抑制。这些形态学和生化的证据表明，上述分裂是独立于染色体复制的。染色体分裂停止和胚胎开始死亡的阶段对应于囊胚的开始，这表明来自胚胎基因组的信息是该物种囊胚形成所必需的。
Development of fertilized eggs of starfish,Asterina pectinifera, was studied in the presence of aphidicolin, a specific inhibitor of eukaryotic DNA synthesis. Fertilized eggs continued to cleave eight or nine times in the presence of aphidicolin, although the delay of development was observed and the size of blastomeres was not equal. The nucleus and chromosomes were undetectable in blastomeres of embryos developed in the presence of aphidicolin, although the amphiaster was clearly visible in the cytoplasm. DNA synthesis of such embryos was nearly completely suppressed. These morphological and biochemical lines of evidence indicate that the above cleavage is independent of chromosomal replication. The stage when achromosomal divisions cease and embryos begin to die corresponds to the onset of blastulation, suggesting that information derived from the embryo genome is required for blastula formation in this species.