A duplex-specific nuclease assisted photoelectrochemical biosensor based on MoS2 @ReS2/Ti3C2 hybrid for ultrasensitive detection of colorectal cancer-related piRNA-31,143
A duplex-specific nuclease assisted photoelectrochemical biosensor based on MoS2 @ReS2/Ti3C2 hybrid for ultrasensitive detection of colorectal cancer-related piRNA-31,143
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DOI：
10.1016/j.actbio.2022.06.037
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发表时间：
2022-08-10
影响因子：
9.7
通讯作者：
Du, Lutao
中科院分区：
文献类型：
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作者：
Li, Juan;Tong, Yao;Du, Lutao
piR-31，143已被鉴定为用于诊断结直肠癌（CRC）的潜在生物标志物。但目前的检测方法操作复杂、成本高，限制了其临床应用。本文报道了一种基于MoS2@ReS2/Ti 3C 2杂化材料和寡核苷酸特异性核酸酶（DSN）辅助信号放大机制的新型光电化学（PEC）生物传感器，用于人血清中皮尔纳-31，143的超灵敏检测。MoS 2和ReS 2之间形成的I型异质结构以及Ti 3C 2的掺杂有助于高的光电流响应。piR-31，143的存在在DSN的辅助下引发了电极表面的链置换反应和酶循环扩增反应，导致复合探针系统的崩溃。因此，PEC生物传感器的光电流与piR-31，143的浓度成比例。PEC生物传感器的线性检测范围为10 - 1 ~ 10 - 6 fM，计算检出限为2 3aM。光电流在15次连续开关照射下的稳定性（相对标准偏差为1.17%）和对piR-31，143的特异性响应证明了PEC生物传感器的可靠性。此外，通过对人血清的批量检测，验证了PEC生物传感器的实用性。用于区分结直肠癌患者和健康对照者的受试者工作特征曲线下面积为0.942，特异性为100%，表明所开发的方法是一种有前途的诊断结直肠癌的方法。用于癌症诊断的piRNA的临床翻译由于繁琐的样品处理和昂贵的仪器而受到检测技术功效的阻碍。在此，我们制作了一个光电化学生物传感器，用于在体外用10 μ L血清超灵敏检测piR-31，143。MoS2@ReS2/Ti 3C 2大大增强了光电流响应，而MoS 2特异性核酸酶提高了检测灵敏度并避免了假阳性。通过改变探针的识别序列，该传感器可以应用于针对不同疾病的多种piRNA检测。此外，电极可以回收利用，有利于降低检测成本。通过适当的自动化和进一步优化，我们的工作可以作为开发准确有效的诊断方法的核心组成部分。(c)2022作者。由Elsevier Ltd代表Acta Materialia Inc.发布这是一篇基于CC BY-NC-ND许可证的开放获取文章（http://creativecommons.org/licenses/by-nc-nd/4.0/）
piR-31,143 has been identified as a potential biomarker for the diagnosis of colorectal cancer (CRC). How-ever, the current detection methods have complicated operations and high cost, which restrict its clini-cal application. In the present work, we reported a new photoelectrochemical (PEC) biosensor based on MoS2 @ReS2 /Ti3C2 hybrid and duplex-specific nuclease (DSN) assisted signal amplification mechanism for ultrasensitive detection of piRNA-31,143 from human serum. The formation of the type I heterostructure between MoS2 and ReS2 and the doping of Ti3C2 contributed to high photocurrent response. The pres-ence of piR-31,143 triggered the chain displacement reaction and enzymatic cyclic amplification reaction on the electrode surface with the assistance of DSN, leading to the collapse of the composite probe sys-tem. Consequently, the photocurrent of the PEC biosensor was proportional to the concentration of piR-31,143. The linear detection range and calculated detection limit of the PEC biosensor were 10 -1 -10 6 fM and 23 aM, respectively. The stability of the photocurrent under 15 consecutive on-off irradiations (with a relative standard deviation of 1.17%) and the specific response to piR-31,143 demonstrated the reliability of the PEC biosensor. In addition, the practicability of the PEC biosensor was verified by batch detection of human serum. The area under the receiver operating characteristic curve used to distinguish CRC pa-tients from healthy controls was 0.942 with 100% specificity, demonstrating the developed method is a promising approach for the diagnosis of CRC. Statement of significance The clinical translation of piRNAs for cancer diagnosis is hindered by efficacy of detection techniques due to tedious sample processing and costly instrumentation. Herein, we fabricated a photoelectrochem-ical biosensor for the ultrasensitive detection of piR-31,143 with 10 mu L serum in vitro. MoS2 @ReS2 /Ti3C2 greatly enhances the photocurrent response while duplex-specific nuclease improves the detection sensi-tivity and avoids false positives. By transforming the recognition sequence of the probe, the sensor can be applied to a variety of piRNAs detection for different diseases. In addition, the electrode can be recycled which is beneficial to reduce the cost of detection. With suitable automation and further optimization, our work may serve as core component in the development of an accurate and efficient diagnosis method. (c) 2022 The Author(s). Published by Elsevier Ltd on behalf of Acta Materialia Inc. This is an open access article under the CC BY-NC-ND license ( http://creativecommons.org/licenses/by-nc-nd/4.0/ )