Differential modulation of large-conductance KCa channels by PKA in pregnant and nonpregnant myometrium.
Differential modulation of large-conductance KCa channels by PKA in pregnant and nonpregnant myometrium.
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PKA 对妊娠和非妊娠子宫肌层大电导 KCa 通道的差异调节。
DOI：
10.1152/ajpcell.1994.266.5.c1459
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
Toro,L
中科院分区：
文献类型：
--
作者：
Perez,G;Toro,L
子宫兴奋性取决于离子通道的活性，其表达受性激素的调节。我们现在表明，蛋白激酶A（PKA）对大电导钙激活K+（KCa）通道活性的作用也取决于激素状态。PKA依赖性磷酸化的重建KCa通道从中孕大鼠通常刺激通道的活性，相反，KCa通道从非妊娠大鼠和人类子宫肌层主要抑制这种机制。这两种作用都是可逆的磷酸酶治疗。这些结果表明，一个重要的因素调节子宫收缩在怀孕期间或定期周期可能是不同的反应KCa通道对PKA诱导的磷酸化。
Uterine excitability depends on ion channel activity, the expression of which is regulated by sexual hormones. We show now that the action of protein kinase A (PKA) on large-conductance calcium-activated K+ (KCa) channel activity also depends on the hormonal status. PKA-dependent phosphorylation of reconstituted KCa channels from midpregnant rats usually stimulated channel activity; in contrast, KCa channels from nonpregnant rat and human myometrium were primarily inhibited by this mechanism. Both effects were reversible by phosphatase treatment. These results suggest that one important factor modulating uterine contractility during pregnancy or the regular cycle may be the differential response of KCa channels toward PKA-induced phosphorylation.