Production of Cycloinulooligosaccharide Fructanotransferase (CFTase) from Bacillus sp. CFCl
Production of Cycloinulooligosaccharide Fructanotransferase (CFTase) from Bacillus sp. CFCl
复制标题
从芽孢杆菌属中生产环低聚果糖转移酶 (CFTase)。
DOI：
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发表时间：
1996
影响因子：
2.8
通讯作者：
Yong
中科院分区：
文献类型：
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作者：
Hwa;Jeonghyun Park;Y. Kwon;Yong
从土壤中分离得到一株能产生胞外环菊寡糖果糖转移酶（CFTase）的菌株CFCl。经鉴定，分离菌株为芽孢杆菌属菌株。发现细菌合成 CFTase 是由添加到培养基中作为碳源的菊粉诱导的。在pH 7.5和分别含有4%菊粉和0.5%蛋白胨作为碳源和氮源的培养基中观察到CFTase的最高活性。在最佳条件下，培养96 h后，培养上清液中酶活力达到最高水平，为85 munits/ml。
A bacterial strain CFCl, which produced an extracellular cycloinulooligosaccharide fructanotransferase(CFTase), was isolated from soil. The isolated strain was identified as a strain of Bacillus sp. The synthesis of CFTase by the bacterium was found to be induced by inulin which was added to the culture medium as a carbon source. The highest activity of CFTase was observed at pH 7.5 and in the medium containing 4% inulin and 0.5% peptone as a carbon source and a nitrogen source, respectively. Under the optimal conditions, the enzyme activity in the culture supernatant reached the highest level of 85 munits/ml after 96 h cultivation.