Coronavirus reverse genetic systems: infectious clones and replicons.
Coronavirus reverse genetic systems: infectious clones and replicons.
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DOI：
10.1016/j.virusres.2014.05.026
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发表时间：
2014-08-30
期刊：
影响因子：
5
通讯作者：
Enjuanes L
中科院分区：
文献类型：
--
作者：
Almazán F;Sola I;Zuñiga S;Marquez-Jurado S;Morales L;Becares M;Enjuanes L
综述和讨论了针对冠状病毒开发的四种不同的反向遗传系统。这些系统是基于使用BACs，体外结扎和牛痘病毒作为载体。冠状病毒反向遗传学是研究冠状病毒复制、病毒-宿主相互作用和发病机制的重要工具。这些反向遗传系统允许开发基因确定的疫苗和分析抗病毒药物。冠状病毒（cov）感染人类和许多动物物种，并与呼吸道、肠道、肝脏和中枢神经系统疾病有关。冠状病毒基因组的大尺寸和某些冠状病毒复制酶基因序列在细菌中繁殖过程中的不稳定性，严重阻碍了类似于用于较小阳性意义RNA病毒的反向遗传系统的发展。为了克服这些限制，在过去的13年中，已经建立了几种替代传统的基于质粒的方法。在本报告中，我们简要回顾和讨论了针对冠状病毒开发的不同反向遗传系统，特别关注严重急性呼吸综合征冠状病毒（SARS-CoV）。
The four different reverse genetic systems developed for CoVs are reviewed and discussed. These systems were based on the use of BACs, in vitro ligation and vaccinia virus as a vector. CoV reverse genetics is an essential tool for the study of CoV replication, virus–host interaction and pathogenesis. These reverse genetic systems allow the development of genetically defined vaccines and the analysis of antiviral drugs. Coronaviruses (CoVs) infect humans and many animal species, and are associated with respiratory, enteric, hepatic, and central nervous system diseases. The large size of the CoV genome and the instability of some CoV replicase gene sequences during its propagation in bacteria, represent serious obstacles for the development of reverse genetic systems similar to those used for smaller positive sense RNA viruses. To overcome these limitations, several alternatives to more conventional plasmid-based approaches have been established in the last 13 years. In this report, we briefly review and discuss the different reverse genetic systems developed for CoVs, paying special attention to the severe acute respiratory syndrome CoV (SARS-CoV).