Natural Wolbachia infections are common in the major malaria vectors in Central Africa
Natural Wolbachia infections are common in the major malaria vectors in Central Africa
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DOI：
10.1111/eva.12804
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发表时间：
2019-09-01
影响因子：
4.1
通讯作者：
Paupy, Christophe
中科院分区：
文献类型：
--
作者：
Ayala, Diego;Akone-Ella, Ousman;Paupy, Christophe
在过去的十年中，内共生细菌沃尔巴克氏体已经成为一种用于病媒疾病控制的生物工具。然而，长期以来，人们认为在按蚊自然种群中不存在沃尔巴克氏体。最近发现冈比亚按蚊复合体内的物种在自然条件下宿主沃尔巴克氏体，为非洲疟疾控制研究提供了新的机会。在这里，我们调查了在加蓬（中非）的25种非洲按蚊的沃尔巴克氏体感染的患病率和多样性。我们的研究结果显示，沃尔巴克氏体存在于这些物种中的16个，包括该地区的主要疟疾病媒。不同物种间的感染率差异很大，证实了样本量是检测感染的关键因素。此外，我们的测序和系统发育分析显示了感染按蚊的沃尔巴克氏体菌株的重要多样性。协同进化分析揭示了一些按蚊种内沃尔巴克氏体传播的模式，表明过去的独立收购事件之后，共同的分支。感染按蚊自然种群的沃尔巴克氏体菌株的多样性为选择合适的表型以抑制疟原虫传播和/或操纵按蚊繁殖提供了一个有希望的机会，这反过来又可用于减少非洲的疟疾负担。
During the last decade, the endosymbiont bacterium Wolbachia has emerged as a biological tool for vector disease control. However, for long time, it was believed that Wolbachia was absent in natural populations of Anopheles. The recent discovery that species within the Anopheles gambiae complex host Wolbachia in natural conditions has opened new opportunities for malaria control research in Africa. Here, we investigated the prevalence and diversity of Wolbachia infection in 25 African Anopheles species in Gabon (Central Africa). Our results revealed the presence of Wolbachia in 16 of these species, including the major malaria vectors in this area. The infection prevalence varied greatly among species, confirming that sample size is a key factor to detect the infection. Moreover, our sequencing and phylogenetic analyses showed the important diversity of Wolbachia strains that infect Anopheles. Co-evolutionary analysis unveiled patterns of Wolbachia transmission within some Anopheles species, suggesting that past independent acquisition events were followed by co-cladogenesis. The large diversity of Wolbachia strains that infect natural populations of Anopheles offers a promising opportunity to select suitable phenotypes for suppressing Plasmodium transmission and/or manipulating Anopheles reproduction, which in turn could be used to reduce the malaria burden in Africa.