Evidence for two suppressor factors secreted by a single cell suggests a solution to the J-locus paradox.
Evidence for two suppressor factors secreted by a single cell suggests a solution to the J-locus paradox.
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单个细胞分泌两种抑制因子的证据表明 J 基因座悖论的解决方案。
DOI：
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发表时间：
1983
影响因子：
11.1
通讯作者：
J. Klein
中科院分区：
文献类型：
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作者：
Z. Ikezawa;C. Baxevanis;B. Arden;ToMio TADAt;Carl R. WALTENBAUGHt;Z. A. Nagy;J. Klein
用乳酸脱氢酶B（LDH-B）致敏的B10.A（2 R）小鼠抑制性T细胞（Ts）与BW 5147胸腺瘤融合产生的杂交瘤可分泌两种T细胞抑制因子（TsF）：TsF-A和TsF-E。TsF-A抑制A β限制性和TsF-E，E β限制性辅助T（Th）细胞。这两种因子中的每一种都由两条多肽链组成，即抗原结合链（ABC）和主要组织相容性复合体（MHC）链。ABC结合LDH-B，然后被Th细胞的两个受体之一识别，并且在因子和Th细胞之间形成抗原桥。这条链在两个因素中大概是相同的。TsF-A的MHC链携带被三组单克隆抗体识别的抗原决定簇：A β链特异性抗体、T细胞中表达并由A β-E β染色体片段控制的II类决定簇特异性抗体以及与J决定簇交叉反应的抗体。TsF-E的MHC链携带E β特异性和J特异性抗体识别的决定簇。这些血清学可检测的决定簇中只有一部分位于Th细胞识别的TsF分子区域。这些发现表明，J决定簇由修饰的E β和修饰的A β链携带。
The hybridoma produced by the fusion of lactate dehydrogenase-B (LDH-B)-primed B10.A(2R) mouse suppressor T (Ts) cells with the BW5147 thymoma secretes two kinds of T suppressor factors (TsF), TsF-A and TsF-E. The TsF-A suppresses A beta-restricted and the TsF-E, E beta-restricted helper T (Th) cells. Each of the two factors consists of two polypeptide chains, an antigen-binding chain (ABC) and a major histocompatibility complex (MHC) chain. The ABC binds LDH-B, which is then recognized by one of the two receptors of the Th cell and an antigen bridge is formed between the factor and the Th cell. This chain is presumably identical in both factors. The MHC chain of the TsF-A carries antigenic determinants recognized by three sets of monoclonal antibodies: antibodies specific for the A beta chain, antibodies specific for class II determinants expressed in T cells and controlled by the A beta-E beta chromosomal segment, and antibodies crossreacting with J determinants. The MHC chain of the TsF-E carries determinants recognized by E beta-specific and by J-specific antibodies. Only some of these serologically detectable determinants reside in the region of the TsF molecule recognized by Th cells. These findings suggest that the J determinants are carried by the modified E beta and also by the modified A beta chains.