CELL-SPECIFIC AND STAGE-SPECIFIC EXPRESSION OF VITAMIN-D RECEPTOR AND CALBINDIN GENES IN RAT INCISOR - REGULATION BY 1,25-DIHYDROXYVITAMIN-D(3)
CELL-SPECIFIC AND STAGE-SPECIFIC EXPRESSION OF VITAMIN-D RECEPTOR AND CALBINDIN GENES IN RAT INCISOR - REGULATION BY 1,25-DIHYDROXYVITAMIN-D(3)
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DOI：
10.1006/dbio.1993.1016
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发表时间：
1993-01-01
影响因子：
2.7
通讯作者：
DUPRET, JM
中科院分区：
文献类型：
--
作者：
BERDAL, A;HOTTON, D;DUPRET, JM
为了探讨1，25-二羟基维生素D3[1，25(OH)2D3]在矿化组织中的作用程度及其与Calbindin基因表达的关系，我们用不同的探针分析了大鼠切牙，包括维生素D受体(VDR)抗体和针对大鼠Calbindin-D9K和Calbindin-D28K的特异性DNA。通过成釉细胞和成牙本质细胞mRNA的Northern印迹分析，研究Calbindin基因表达的发育和激素控制。用光镜免疫细胞化学方法研究VDR的分布和激素诱导的变化。在不断萌出的门牙的显微解剖部分观察到不同组织和阶段特异性的钙结合蛋白基因的表达。这两种钙结合蛋白在成釉细胞中均有表达，而在成牙本质细胞中只有Calbindln-D29K表达。此外，在成釉细胞中，Calbindin-D29K的表达先于Calbindin-D9K的表达。VDR的免疫反应性在所有的前体细胞中都存在，并在分化过程中逐渐降低，而在分化的组织中，激素的上调仅限于硬组织形成细胞，即成釉细胞和成牙本质细胞。此外，Calbindin基因的表达似乎受1，25(OH)2D3的调控。综上所述，这些数据表明成釉细胞和成牙本质细胞是1，25(OH)2D3的靶细胞，并为牙齿发育过程中激素控制提供了第一个洞察力。
To investigate the extent of 1,25-dihydroxyvitamin D3[1,25(OH)2D3] action and its relationships to calbindin gene expression in mineralized tissues, we have analyzed rat incisors with different probes, including a vitamin D receptor (VDR) antibody and specific cDNAs to rat calbindin-D9Kand calbindin-D28K. Developmental and hormonal controls of calbindin gene expression were investigated by Northern blot analysis of ameloblast and odontoblast mRNA. Distribution and hormone-induced changes of VDR were also studied by light microscopic immunocytochemistry. A differential tissue- and stage-specific expression of the calbindin genes was observed in microdissected portions of the continuously erupting incisor. The two calbindins were expressed in ameloblasts, whereas only calbindln-D29Kwas expressed in odontoblasts. Moreover, in ameloblasts, expression of calbindin-D29Kpreceded that of calbindin-D9K. Immunoreactivity for VDR was present in all progenitor cells and progressively decreased during the differentiation process, whereas, in differentiated tissues, a hormonal upregulation was restricted to hard tissue-forming cells, i.e., ameloblasts and odontoblasts. Furthermore, calbindin gene expression appeared to be regulated by 1,25(OH)2D3. Taken together, these data indicate that ameloblasts and odontoblasts are target cells for 1,25(OH)2D3and provide the first insights into the hormonal control of tooth genes during development.