Proteasomal activity in skeletal muscle: a matter of assay design, muscle type, and age.
Proteasomal activity in skeletal muscle: a matter of assay design, muscle type, and age.
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DOI：
10.1016/j.ab.2009.12.026
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发表时间：
2010-04
影响因子：
2.9
通讯作者：
K. Strucksberg;Karthikeyan Tangavelou;R. Schröder;C. Clemen
中科院分区：
文献类型：
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作者：
K. Strucksberg;Karthikeyan Tangavelou;R. Schröder;C. Clemen
泛素-蛋白酶体系统（UPS）是调节蛋白和错误折叠蛋白的主要降解系统。UPS功能已被暗示在各种人类疾病的发病机制中发挥核心作用。由于生化分析往往受到可用的患病组织的量，我们报告的建立和验证的发光为基础的蛋白酶体活性测定适用于5毫克量的骨骼肌。我们证明，具体的蛋白酶体活性不同，在个别肌肉群，并随着年龄的增长而下降。这些发现保证了在哺乳动物骨骼肌病理学中使用适当的对照和仔细解释结果。
The ubiquitin–proteasome system (UPS) is a major degradation system for regulatory and misfolded proteins. UPS function has been implicated to exert a central role in the pathogenesis of various human diseases. Because biochemical analyses are often hampered by the amount of available diseased tissue, we report on the establishment and validation of a luminescence-based proteasomal activity assay applicable to 5-mg quantities of skeletal muscle. We demonstrate that the specific proteasomal activity differs in individual muscle groups and decreases with aging. These findings warrant the use of appropriate controls and a careful interpretation of results in mammalian skeletal muscle pathologies.