Atomic force microscopy of biological samples at low temperature
Atomic force microscopy of biological samples at low temperature
复制标题
低温生物样品的原子力显微镜
DOI：
10.1116/1.585442
复制
发表时间：
1991
影响因子：
1.4
通讯作者：
P. Hansma
中科院分区：
文献类型：
--
作者：
C. Prater;M. Wilson;J. Garnaes;J. Massie;V. Elings;P. Hansma
原子力显微镜(AFM)已经成功地对刚性样品进行了原子分辨率的成像，但这种分辨率在室温下的软生物样品上是无法比拟的。在许多生物样本上，即使是小到10−9N的跟踪力也会在扫描过程中使样本扭曲。在低温下，蛋白质冻结成单一构象状态，变得更加坚硬。在本文中，我们论证了用低温原子力显微镜对冷冻生物样品进行原子分辨是可行的。我们还展示了一些初步的低温原子力显微镜图像的生物样品，紫膜。
The atomic force microscope (AFM) has been successful imaging rigid samples with atomic resolution, but this resolution has not been matched on soft biological samples at room temperature. On many biological samples even tracking forces as small as 10−9 N distort the sample during a scan.At low temperatures proteins freeze into a single conformational state and become much more rigid. In this paper we demonstrate that it should be feasible to get atomic resolution on frozen biological samples with a low‐temperature AFM. We also show some preliminary low‐temperature AFM images of one biological sample, purple membrane.