Assessing the conservation of mammalian gene expression using high-density exon arrays
Assessing the conservation of mammalian gene expression using high-density exon arrays
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DOI：
10.1093/molbev/msm061
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发表时间：
2007-06-01
影响因子：
10.7
通讯作者：
Wong, Wing Hung
中科院分区：
文献类型：
--
作者：
Xing, Yi;Ouyang, Zhengqing;Wong, Wing Hung
来自多个物种的微阵列数据已被用于研究基因表达的进化约束。来自常规微阵列平台如3'表达阵列的表达测量受到平台依赖性探针效应的强烈影响，所述平台依赖性探针效应可能在物种之间引入明显但误导的差异。在本文中，我们使用来自高密度外显子阵列平台的数据评估了成年组织中哺乳动物基因表达的保守性。外显子阵列在单个阵列上具有超过600万个探针，靶向基因组中的所有外显子。我们发现，与3'阵列数据不同，外显子阵列的基因表达测量揭示了人类和小鼠在多种组织中高度保守的基因表达模式。我们的分析提供了强有力的证据，广泛的稳定选择压力在哺乳动物进化过程中的转录丰度。
Microarray data from multiple species have been used to study evolutionary constraints on gene expression. Expression measurements from conventional microarray platforms such as the 3' expression arrays are strongly affected by platform-dependent probe effects that may introduce apparent but misleading discrepancies between species. In this manuscript, we assess the conservation of mammalian gene expression in adult tissues using data from a high-density exon array platform. The exon arrays have more than 6 million probes on a single array targeting all exons in a genome. We find that, unlike 3' array data, gene expression measurements from exon arrays reveal patterns of gene expression that are highly conserved between humans and mice in multiple tissues. Our analysis provides strong evidence for widespread stabilizing selection pressure on transcript abundance during mammalian evolution.