Murine B7-2, an alternative CTLA4 counter-receptor that costimulates T cell proliferation and interleukin 2 production.
Murine B7-2, an alternative CTLA4 counter-receptor that costimulates T cell proliferation and interleukin 2 production.
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DOI：
10.1084/jem.178.6.2185
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发表时间：
1993-12-01
期刊：
影响因子：
--
通讯作者：
Laszlo G
中科院分区：
文献类型：
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作者：
Freeman GJ;Borriello F;Hodes RJ;Reiser H;Gribben JG;Ng JW;Kim J;Goldberg JM;Hathcock K;Laszlo G
在抗原呈递细胞（APC）上表达的B7 - 1分子为T细胞活化提供了关键的共刺激信号。近期研究表明，在小鼠和人类中存在其他非B7 - 1的CTLA4反受体。在此，我们描述了小鼠B7 - 2（mB7 - 2）基因的分子克隆，并证明了其共刺激功能。小鼠B7 - 2 cDNA编码免疫球蛋白超基因家族的一个成员，它可与CTLA4 - Ig结合，能用GL1染色，但不能用抗mB7 - 1单克隆抗体染色。小鼠B7 - 2共刺激CD4⁺T细胞的增殖和白细胞介素2的产生，并且这种共刺激可被CTLA4 - Ig或GL1单克隆抗体抑制。B7 - 2分子的鉴定将允许对B7：CD28/CTLA4共刺激途径进行进一步的调控，该途径已被证明与预防免疫耐受、诱导肿瘤免疫以及最近与自身免疫的发病机制有关。
The B7-1 molecule, expressed on antigen presenting cells (APC), provides a crucial costimulatory signal for T cell activation. Recent studies demonstrate the existence of alternative, non-B7-1 CTLA4 counter-receptors in mice and humans. Here, we describe the molecular cloning and demonstrate costimulatory function of the murine B7-2 (mB7- 2) gene. Murine B7-2 cDNA encodes a member of the Ig supergene family that binds CTLA4-Ig and stains with the GL1 but not anti-mB7-1 mAb. Murine B7-2 costimulates the proliferation and interleukin 2 production of CD4+ T cells and this costimulation can be inhibited by either CTLA4- Ig or GL1 mAb. Identification of the B7-2 molecule will permit further manipulation of the B7:CD28/CTLA4 costimulatory pathway which has been shown to be involved in the prevention of tolerance, induction of tumor immunity, and most recently, in the pathogenesis of autoimmunity.