2 NUCLEAR MUTATIONS DISRUPT DISTINCT PATHWAYS FOR TARGETING PROTEINS TO THE CHLOROPLAST THYLAKOID
2 NUCLEAR MUTATIONS DISRUPT DISTINCT PATHWAYS FOR TARGETING PROTEINS TO THE CHLOROPLAST THYLAKOID
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DOI：
10.1002/j.1460-2075.1995.tb00062.x
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发表时间：
1995-08-15
期刊：
影响因子：
11.4
通讯作者：
BARKAN, A
中科院分区：
文献类型：
--
作者：
VOELKER, R;BARKAN, A
体外实验结果表明，核编码蛋白至少存在三种靶向叶绿体类囊体膜的途径，但目前已鉴定出的靶向机制成分很少，而且这三种途径之间的关系尚不清楚。为了研究类囊体蛋白靶向的机制，我们鉴定了玉米中导致靶向缺陷的核突变，我们发现了两种突变，tha1和hcf106，它们破坏了不同组蛋白在类囊体腔内的定位，tha1突变干扰了一种叶绿体编码蛋白、细胞色素f和三种核编码蛋白、质体蓝素、psaF基因产物和33 kDa亚基的靶向。放氧复合物。 hcf106 突变干扰释氧复合物 16 和 23 kDa 亚基的靶向。tha1 和 hcf106 表型提供了第一个体内证据，支持两种不同的类囊体靶向途径的存在。它们的表型还提供了证据，表明一种叶绿体编码的蛋白质细胞色素f参与“tha1”途径，表明核和叶绿体编码的蛋白质可以针对常见的机制。
Results of in vitro experiments have suggested the existence of at least three pathways by which nuclear-encoded proteins are targeted to the chloroplast thylakoid membrane, However, few components of the targeting machinery have been identified and the relationship between the three pathways is not clear. To investigate mechanisms underlying thylakoid protein targeting, we identified nuclear mutations in maize that cause targeting defects, We found two mutations, tha1 and hcf106, that disrupt the localization of different sets of proteins to the thylakoid lumen, The tha1 mutation interferes with the targeting of one chloroplast-encoded protein, cytochrome f, and three nuclear-encoded proteins, plastocyanin, the psaF gene product and the 33 kDa subunit of the oxygen-evolving complex. The hcf106 mutation interferes with the targeting of the 16 and 23 kDa subunits of the oxygen-evolving complex, The tha1 and hcf106 phenotypes provide the first in vivo evidence supporting the existence of two distinct thylakoid-targeting pathways. Their phenotypes also provide evidence that one chloroplast-encoded protein, cytochrome f, engages the 'tha1' pathway, indicating that nuclear- and chloroplast-encoded proteins can be targeted common machinery.