CC chemokine ligand 7 expression in liver metastasis of colorectal cancer
CC chemokine ligand 7 expression in liver metastasis of colorectal cancer
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DOI：
10.3892/or.2012.1815
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发表时间：
2012-08-01
期刊：
影响因子：
4.2
通讯作者：
Lee, Kuhn Uk
中科院分区：
文献类型：
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作者：
Cho, Yong Beom;Lee, Woo Yong;Lee, Kuhn Uk
结直肠癌（CRC）患者死亡的主要原因是在远离原发肿瘤的部位发生转移性病变。因此，寻找与肿瘤转移相关的生物标志物并研究其可能的机制具有重要意义。最近的数据表明，称为趋化因子的可溶性引诱分子支持某些癌症向某些器官的转移。为了鉴定在CRC的肝转移中差异表达的分子调节剂，进行PCR阵列分析，CC趋化因子配体7（CCL7）在肝转移肿瘤组织中显示出显著的过表达。为了验证PCR阵列的结果，选择了30例原发性CRC和肝转移患者。免疫组化和实时荧光PCR分析显示，CCL7在正常结肠上皮中表达，肝转移癌中的表达高于原发性大肠癌（P
The main cause of death for colorectal cancer (CRC) patients is the development of metastatic lesions at sites distant from the primary tumor. Therefore, it is important to find biomarkers that are related to the metastasis and to study the possible mechanisms. Recent data have shown that soluble attractant molecules called chemokines support the metastasis of certain cancers to certain organs. To identify molecular regulators that are differentially expressed in liver metastasis of CRC, PCR array analysis was performed and CC chemokine ligand 7 (CCL7) showed remarkable overexpression in liver metastatic tumor tissues. To validate the results of the PCR array, 30 patients with primary CRC and liver metastases were selected. Immunohistochemistry and real-time PCR analysis showed that CCL7 was expressed in normal colonic epithelium and the expression was higher in liver metastases compared to primary CRC (P