Toxicity of copper-based dental alloys in cell culture.
Toxicity of copper-based dental alloys in cell culture.
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细胞培养中铜基牙科合金的毒性。
DOI：
10.1002/jbm.820231002
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
Arabella B. Tilden
中科院分区：
文献类型：
--
作者：
J. Bumgardner;Linda C. Lucas;Arabella B. Tilden
研究了三种商业铜基牙科铸造合金-Duracast MS，Goldent和Trindium-三种实验铜合金和对照金合金Modulay的生物相容性。Trindium、Duracast MS、实验合金1和2是铝青铜; Goldent是混合铝-黄铜合金;并且实验合金#3是高锌黄铜合金。ASTM F813-83医疗器械材料直接接触细胞培养评价的标准规范，采用3天直接接触细胞培养方案和原子吸收光谱法评价这些合金在Waymouth和RPMI 1640完全培养基中的生物相容性。还在Waymouth培养基中进行了使用3 H-胸苷摄取的细胞增殖试验。在本研究中，在任一培养基中的ASTM和3天培养试验期间，仅实验合金#3引起成纤维细胞单层的形态和活力改变。暴露于实验合金#3的细胞培养物在Waymouth培养基中经历大于16.0ppm的铜浓度，在RPMI 1640培养基中经历大于10 ppm的铜浓度。还观察到实验合金#3周围的细胞毒性区大小的差异，其中较大的区域出现在Waymouth培养基中。与直接细胞接触研究相反，与对照组相比，所有合金在金属离子浓度大大降低的情况下都会导致Waymouth培养基中3 H-胸苷吸收的减少。因此，DNA合成的不利变化发生在低得多的铜和锌浓度比形态和活力的变化。因此，生物相容性的评估取决于评价的参数，并且在材料被认为具有生物相容性之前必须分析几个参数。
The biocompatibility of three commercial copper-based dental casting alloys--Duracast MS, Goldent, and Trindium--three experimental copper alloys, and a control gold alloy, Modulay, were investigated. Trindium, Duracast MS, experimental alloys 1 and 2 are aluminum bronzes; Goldent is a hybrid aluminum-brass alloy; and experimental alloy #3 is a high zinc brass alloy. ASTM F813-83 Standard Practice for Direct Contact Cell Culture Evaluation of Materials for Medical Devices, a 3-day direct-contact cell culture regimen and atomic absorption spectroscopy were utilized for evaluating the biocompatibility of these alloys in both Waymouth's and RPMI 1640 complete media. Cellular proliferation assays, using 3H-thymidine uptake, were also conducted in Waymouth's media. In this investigation, only the experimental alloy #3 elicited alterations in morphology and viability of the fibroblast monolayer during the ASTM and 3-day culture tests in either media. Cell cultures exposed to experimental alloy #3 experienced copper concentrations greater than 16.0 ppm in Waymouth's and 10 ppm copper in RPMI 1640 media. Differences in the size of the cytotoxic zone around experimental alloy #3 were also observed, with the larger zone occurring in Waymouth's media. In contrast to the direct cell contact studies, all alloys caused decreases in 3H-thymidine uptake in Waymouth's media at much reduced metal ion concentrations as compared to the controls. Thus, adverse changes in DNA synthesis occurred at much lower copper and zinc concentrations than changes in morphology and viability. Consequently, the assessment of biocompatibility is dependent on the parameters evaluated, and several parameters must be analyzed before a material may be considered biocompatible.