Microbial contamination in four commercially available denture adhesives.
Microbial contamination in four commercially available denture adhesives.
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四种市售义齿粘合剂中的微生物污染。
DOI：
10.1016/0022-3913(94)90024-8
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
W. D. Gates
中科院分区：
文献类型：
--
作者：
M. Leusch;W. D. Gates
四个品牌的市售粉末义齿粘合剂进行了微生物污染测试。用0.5 gm每种粘合剂样品接种含有脑心浸液（BHI）或沙氏（SAB）琼脂的无菌培养皿。将BHI板在35 ° C下有氧和厌氧孵育2周，并将SAB板在30 °C下有氧孵育2周。通过孵育未接种的对照平板确认这些程序的无菌性。所有测试的粉末义齿粘合剂瓶都含有微生物。观察到细菌和真菌生长。然后使粘合剂容器经受10分钟的微波能量（650 W）以将粘合剂材料灭菌。重新测试后，将任何仍被污染的瓶子再进行10分钟的微波暴露，然后重新测试。在暴露于微波能量后，24个瓶子中的7个仍然显示出微生物污染的证据。在这七瓶酒中，有五瓶是同一个牌子。Fisher精确检验显示，该品牌与其他品牌之间的污染样品百分比存在显著差异（p< 0.05）。微波能量减少了所有样品中的微生物污染，但未能完全灭菌24个样品中的7个。
Four brands of commercially available powder denture adhesives were tested for microbial contamination. Sterile Petri plates that contained either brain-heart infusion (BHI) or Sabouraud's (SAB) agar were inoculated with 0.5 gm samples of each adhesive. The BHI plates were incubated aerobically and anaerobically at 35 ° C and the SAB plates aerobically at 30 °C for 2 weeks. The sterility of these procedures was confirmed by incubating uninoculated control plates. All bottles of powder denture adhesive tested contained microorganisms. Both bacterial and fungal growth were noted. The containers of adhesive were then subjected to 10 minutes of microwave energy (650 W) to sterilize the adhesive material. After retesting, any bottles still contaminated were subjected to additional 10 minutes of microwave exposure and then retested. Seven of 24 bottles still showed evidence of microbial contamination after exposure to microwave energy. Of the seven bottles, five were of one brand. A Fisher's exact test showed significant differences in the percentage of contaminated samples between that and the other brands (p< 0.05). Microwave energy reduced microbial contamination in all samples but failed to completely sterilize seven of 24 samples.