Propofol Anesthesia Alters Spatial and Topologic Organization of Rat Brain Metabolism
Propofol Anesthesia Alters Spatial and Topologic Organization of Rat Brain Metabolism
复制标题
异丙酚麻醉改变大鼠脑代谢的空间和拓扑组织
DOI：
10.1097/aln.0000000000002876
复制
发表时间：
2019-10-01
期刊：
影响因子：
8.8
通讯作者：
Zhang, Jun
中科院分区：
文献类型：
--
作者：
Chen, Yali;Bao, Weiqi;Zhang, Jun
背景：麻醉期间意识丧失降低了局部和整体脑葡萄糖代谢率。尽管如此，几乎没有研究过麻醉诱导的逐渐变化对整个大脑代谢网络中意识的影响。本研究的目的是确定静脉麻醉剂异丙酚诱导的不同意识/麻醉状态的特定脑代谢模式特征。方法：在不同时间，20只Sprague-Dawley成年大鼠静脉注射三种不同剂量的异丙酚，诱导不同的麻醉状态：轻度镇静（20 mg.kg（-1）.h（-1）），深度镇静（40 mg.kg（-1）.h（-1）），和深度麻醉（80 mg.kg（-1）.h（-1））。使用[F-18]氟脱氧葡萄糖正电子发射断层扫描脑成像，代谢分布和代谢地形图的空间模式的变化进行了研究，通过应用基于体素的空间协方差分析和图论analysis.Results：明显减少，发现在基线代谢沿着与丙泊酚诱导的麻醉过程中改变代谢空间分布。此外，图论分析显示，在丙泊酚诱导的深度麻醉期间，代谢脑网络的整体和局部效率被破坏，其特征在于代谢连接和能量效率降低（轻度镇静总体效率/局部效率= 0.6985/0.7190，深度镇静总体效率/局部效率= 0.7444/0.7875，深度麻醉总体有效率/局部有效率= 0.4498/0.6481;轻度镇静与深度镇静，总体有效率：P = 0.356，局部有效率：P = 0.079;轻度镇静与深度麻醉，总体有效率：P < 0.0001，局部有效率：P < 0.0001;深度镇静与深度麻醉，整体效率：P < 0.0001，局部效率：P < 0.0001）。脑代谢和代谢连接强度之间也存在较强的空间相关性，随着麻醉水平的加深而显著降低（相关系数：轻度镇静，r = 0.55，深度镇静，r = 0.47;深度麻醉，r = 0.23;镇静组与深度麻醉组之间P < 0.0001）。这些数据揭示了麻醉相关的代谢脑网络的空间和拓扑结构的改变，以及异丙酚麻醉期间代谢连接和脑代谢之间的密切关系。这些发现可能为麻醉诱导意识丧失的代谢机制提供新的见解。
Background: Loss of consciousness during anesthesia reduces local and global rate of cerebral glucose metabolism. Despite this, the influence of gradual anesthetic-induced changes on consciousness across the entire brain metabolic network has barely been studied. The purpose of the present study was to identify specific cerebral metabolic patterns characteristic of different consciousness/anesthesia states induced by intravenous anesthetic propofol.Methods: At various times, 20 Sprague-Dawley adult rats were intravenously administered three different dosages of propofol to induce different anesthetic states: mild sedation (20 mg.kg(-1).h(-1)), deep sedation (40 mg.kg(-1).h(-1)), and deep anesthesia (80 mg.kg(-1).h(-1)). Using [F-18]fluorodeoxyglucose positron emission tomography brain imaging, alterations in the spatial pattern of metabolic distribution and metabolic topography were investigated by applying voxel-based spatial covariance analysis and graph-theory analysis.Results: Evident reductions were found in baseline metabolism along with altered metabolic spatial distribution during propofol-induced anesthesia. Moreover, graph-theory analysis revealed a disruption in global and local efficiency of the metabolic brain network characterized by decreases in metabolic connectivity and energy efficiency during propofol-induced deep anesthesia (mild sedation global efficiency/local efficiency = 0.6985/0.7190, deep sedation global efficiency/local efficiency = 0.7444/0.7875, deep anesthesia global efficiency/local efficiency = 0.4498/0.6481; mild sedation vs. deep sedation, global efficiency: P = 0.356, local efficiency: P = 0.079; mild sedation vs. deep anesthesia, global efficiency: P < 0.0001, local efficiency: P < 0.0001; deep sedation vs. deep anesthesia, global efficiency: P < 0.0001, local efficiency: P < 0.0001). A strong spatial correlation was also found between cerebral metabolism and metabolic connectivity strength, which decreased significantly with deepening anesthesia level (correlation coefficients: mild sedation, r = 0.55, deep sedation, r = 0.47; deep anesthesia, r = 0.23; P < 0.0001 between the sedation and deep anesthesia groups).Conclusions: The data revealed anesthesia-related alterations in spatial and topologic organization of metabolic brain network, as well as a close relationship between metabolic connectivity and cerebral metabolism during propofol anesthesia. These findings may provide novel insights into the metabolic mechanism of anesthetic-induced loss of consciousness.