IMMUNOHISTOCHEMICAL LOCALIZATION OF BASEMENT-MEMBRANE COMPONENTS IN PSEUDOEXFOLIATION MATERIAL OF THE LENS CAPSULE
IMMUNOHISTOCHEMICAL LOCALIZATION OF BASEMENT-MEMBRANE COMPONENTS IN PSEUDOEXFOLIATION MATERIAL OF THE LENS CAPSULE
复制标题
DOI：
10.3109/02713689209001788
复制
发表时间：
1992-04-01
影响因子：
2
通讯作者：
NAUMANN, GOH
中科院分区：
文献类型：
--
作者：
SCHLOTZERSCHREHARDT, U;DORFLER, S;NAUMANN, GOH
假性剥脱（PSX）综合征长期以来被推测是一种基底膜代谢紊乱的疾病。为了检验这个概念，我们通过免疫荧光和电子显微镜免疫金技术研究了 30 个晶状体前囊的囊前 PSX 沉积物中所有主要基底膜成分的存在。我们已经证明硫酸乙酰肝素和硫酸软骨素蛋白聚糖、层粘连蛋白、巢蛋白/巢蛋白、纤连蛋白和淀粉样蛋白 P 是 PSX 材料的组成部分； IV 型胶原仅限于介于囊表面和典型 PSX 材料之间的微纤维层。弹性蛋白表位的额外存在表明 PSX 材料是无序细胞外基质合成的多组分表达，包括主要非胶原基底膜组分的掺入。 PSX 材料对硫酸软骨素的广泛标记表明糖胺聚糖的过量产生和代谢异常是这种疾病的关键变化之一。
The pseudoexfoliation (PSX) syndrome has long been speculated to be a disorder of disturbed basement membrane metabolism. To test this concept, we investigated the presence of all principal basement membrane components in precapsular PSX deposits of 30 anterior lens capsules by immunofluorescence and electron microscopic immunogold techniques. We have shown heparan sulfate and chondroitin sulfate proteoglycans, laminin, entactin/nidogen, fibronectin, and amyloid P protein to be an integral constituent of PSX material; type IV collagen was restricted to a microfibrillar layer interposed between capsular surface and typical PSX material. The additional presence of elastin epitopes indicates that the PSX material is a multicomponent expression of a disordered extracellular matrix synthesis including the incorporation of the principal noncollagenous basement membrane components. The extensive labelling of PSX material for chondroitin sulfate suggests an overproduction and abnormal metabolism of glycosaminoglycans to be one of the key changes in this disorder.