Microbial community structure and diversity within hypersaline Keke Salt Lake environments
Microbial community structure and diversity within hypersaline Keke Salt Lake environments
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高盐科科盐湖环境中微生物群落结构和多样性
DOI：
10.1139/cjm-2016-0773
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发表时间：
2017-11-01
影响因子：
2.8
通讯作者：
Zhu, Derui
中科院分区：
文献类型：
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作者：
Han, Rui;Zhang, Xin;Zhu, Derui
可可盐湖位于中国柴达木盆地。它是一个独特的硫酸镁亚型高盐湖，具有石盐域生态系统，但其微生物多样性尚未得到研究。在这里，微生物群落结构和多样性进行了研究，通过高通量测序的V3-V5区的16 S rRNA基因。一个高多样性的操作分类单位检测到细菌和细菌（734和747，分别），包括21门，43纲，201属细菌和4门，4纲，39属细菌。与其他高盐度湖泊相比，富盐湖泊中厚壁菌门中的芽孢杆菌属（51.52%~ 58.35%）、乳球菌属（9.52%~ 10.51%）和海洋芽孢杆菌属（8.82%~ 9.88%）占绝对优势（74.81%~ 80.99%）。优势菌属属于盐杆菌科，特别是盐生菌属（Halonotius）、盐生菌属（Halorubellus）、盐生菌属（Halorubrum）、盐生菌属（Halorubrum）和盐单胞菌属（Natronomonas）（丰度> 10%）。此外，我们报告的存在Nanohalaarchaeota和Woesearchaeota在青藏高原湖泊，这是以前没有记录。总盐度（特别是Mg 2+，Cl-，Na+和K+）主要与样品的分类分布。这些结果扩展了我们对高盐湖泊内微生物资源利用的理解，以及允许它们栖息在这种环境中的优势微生物的潜在适应。
Keke Salt Lake is located in the Qaidamu Basin of China. It is a unique magnesium sulfate-subtype hypersaline lake that exhibits a halite domain ecosystem, yet its microbial diversity has remained unstudied. Here, the microbial community structure and diversity was investigated via high-throughput sequencing of the V3-V5 regions of 16S rRNA genes. A high diversity of operational taxonomic units was detected for Bacteria and Archaea (734 and 747, respectively), comprising 21 phyla, 43 classes, and 201 genera of Bacteria and 4 phyla, 4 classes, and 39 genera of Archaea. Salt-saturated samples were dominated by the bacterial genera Bacillus (51.52%-58.35% relative abundance), Lactococcus (9.52%-10.51%), and Oceanobacillus (8.82%-9.88%) within the Firmicutes phylum (74.81%-80.99%), contrasting with other hypersaline lakes. The dominant Archaea belonged to the Halobacteriaceae family, and in particular, the genera (with an abundance of > 10% of communities) Halonotius, Halorubellus, Halapricum, Halorubrum, and Natronomonas. Additionally, we report the presence of Nanohaloarchaeota and Woesearchaeota in Qinghai-Tibet Plateau lakes, which has not been previously documented. Total salinity (especially Mg2+, Cl-, Na+, and K+) mostly correlated with taxonomic distribution across samples. These results expand our understanding of microbial resource utilization within hypersaline lakes and the potential adaptations of dominant microorganisms that allow them to inhabit such environments.