Comparison of virulence factors and expression of specific genes between uropathogenic Escherichia coli and avian pathogenic E. coli in a murine urinary tract infection model and a chicken challenge model.
Comparison of virulence factors and expression of specific genes between uropathogenic Escherichia coli and avian pathogenic E. coli in a murine urinary tract infection model and a chicken challenge model.
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DOI：
10.1099/mic.0.024869-0
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发表时间：
2009-05
期刊：
影响因子：
1.5
通讯作者：
Lixiang Zhao;Song Gao;Haixia Huan;Xiaojing Xu;Xiaoping Zhu;Weixia Yang;Q. Gao;Xiufan Liu
中科院分区：
文献类型：
--
作者：
Lixiang Zhao;Song Gao;Haixia Huan;Xiaojing Xu;Xiaoping Zhu;Weixia Yang;Q. Gao;Xiufan Liu
禽致病性大肠杆菌（APEC）和泌尿系致病性大肠杆菌（E.大肠杆菌（UPEC）在不同宿主的肠外生境中建立感染。由于肠外致病性E.本文对100株APEC分离株和202株UPEC分离株的毒力基因含量和系统发育群进行了比较。这两个群体在血清群、系统发育群和毒力基因型方面表现出大量重叠，包括它们拥有与APEC大的可传播质粒相关的某些基因。在鸡攻击模型中，UPEC U17和APEC E058具有相似的LD（50），表明UPEC U17有可能在家禽中引起重大疾病。为了进一步了解UPEC和APEC之间的相似性，将UPEC U17和APEC E058的152个特异性基因在小鼠尿路感染（UTI）模型和鸡攻击模型中的体内表达与这些菌株在丰富培养基中静态生长至指数期的表达进行比较。结果发现，在同一模型（小鼠UTI或鸡攻击）中，UPEC U17和APEC E058的各种基因显示出相似的表达趋势。几个铁相关基因在UTI模型和/或鸡攻击模型中上调，表明铁的获得对E.大肠杆菌在血液或泌尿道中存活。基于这些结果，APEC作为人类UPEC或作为UPEC毒力基因库的潜力应予以考虑。此外，本研究还比较了体内APEC和UPEC毒力基因的转录谱。
Avian pathogenic Escherichia coli (APEC) and uropathogenic E. coli (UPEC) establish infections in extraintestinal habitats of different hosts. As the diversity, epidemiological sources and evolutionary origins of extraintestinal pathogenic E. coli (ExPEC) are so far only partially defined, in the present study,100 APEC isolates and 202 UPEC isolates were compared by their content of virulence genes and phylogenetic groups. The two groups showed substantial overlap in terms of their serogroups, phylogenetic groups and virulence genotypes, including their possession of certain genes associated with large transmissible plasmids of APEC. In a chicken challenge model, both UPEC U17 and APEC E058 had similar LD(50), demonstrating that UPEC U17 had the potential to cause significant disease in poultry. To gain further information about the similarities between UPEC and APEC, the in vivo expression of 152 specific genes of UPEC U17 and APEC E058 in both a murine urinary tract infection (UTI) model and a chicken challenge model was compared with that of these strains grown statically to exponential phase in rich medium. It was found that in the same model (murine UTI or chicken challenge), various genes of UPEC U17 and APEC E058 showed a similar tendency of expression. Several iron-related genes were upregulated in the UTI model and/or chicken challenge model, indicating that iron acquisition is important for E. coli to survive in blood or the urinary tract. Based on these results, the potential for APEC to act as human UPEC or as a reservoir of virulence genes for UPEC should be considered. Further, this study compared the transcriptional profile of virulence genes among APEC and UPEC in vivo.