Symptomology and etiology of a new disease, yellow stunt, and root rot of standing milkvetch caused by Embellisia sp. in Northern China
Symptomology and etiology of a new disease, yellow stunt, and root rot of standing milkvetch caused by Embellisia sp. in Northern China
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DOI：
10.1007/s11046-007-9002-2
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发表时间：
2007-05
期刊：
影响因子：
5.5
通讯作者：
Y. Li;Z. Nan
中科院分区：
文献类型：
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作者：
Y. Li;Z. Nan
Anbellisiasp.已被确定为引起多年生草本豆科牧草<Standing Milkvetch‘(Astragalus adSurgensPall.)在中国北部，严重降低了沙打旺的种植密度和退化程度。该病在甘肃省的一个试验点很常见，在那里，人们通过茎呈红棕色、叶片呈黄色和坏死状的矮化植株来识别该病。Anbellisiasp.从有症状的茎、叶片、叶柄和根组织中分离到的菌株频率不同，高达90%。单孢子分离物在PCA、PDA、V-8和小麦干草煎液琼脂上生长缓慢。通过接种种子、将2日龄预萌发幼苗浸泡在接种剂中、在6月龄植株上喷洒接种剂来确认致病性。试验植株上的症状包括黄叶病、茎和叶柄褐变、侧枝发育不良、叶黄色、小、扭曲和坏死叶、枯梢病、芽枯病、冠腐病、根腐病和植株死亡。该病定名为沙打旺的黄矮病和根腐病，以区别于其他已知病原菌引起的病害。对中华按蚊也有致病作用，但对其他11种供试植物无致病作用。
AnEmbellisiasp. has been established as the cause of a new disease of the herbaceous perennial forage legume, ‹standing milkvetch’ (Astragalus adsurgensPall.) in Northern China, which severely reduces plant density and degradesA.adsurgensstands. The disease was common at an experimental location in Gansu Province where it was recognized by the occurrence of stunted plants with reddish-brown stems and yellow and necrotic leaf blades. AnEmbellisiasp. was isolated from symptomatic stem, leaf blade, petiole, and root tissues at varying frequencies of up to 90%. Single-spore isolates grew very slowly on PCA, PDA, V-8 and, wheat hay decoction agar. Pathogenicity was confirmed by inoculation of seeds, dipping 2-day-old pre-germinated seedlings in inoculum and spraying inoculum on 6-month-old plants. Symptoms on test plants included yellow leaf lesions, brown lesions on stems and petioles, stunted side-shoots with yellow, small, distorted and necrotic leaves, shoot blight, bud death, crown rot, root rot, and plant death. The disease is named as ‹yellow stunt and root rot’ ofA.adsurgensto distinguish it from diseases caused by other known pathogens.Embellisiasp. is also pathogenic toA.sinicusbut not to 11 other tested plant species.