Cellular and subcellular localization of EFA6C, a third member of the EFA6 family, in adult mouse Purkinje cells
Cellular and subcellular localization of EFA6C, a third member of the EFA6 family, in adult mouse Purkinje cells
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DOI：
10.1111/j.1471-4159.2005.03421.x
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发表时间：
2005-11
影响因子：
4.7
通讯作者：
Shigetsune Matsuya;H. Sakagami;A. Tohgo;Y. Owada;Hye-Won Shin;H. Takeshima;K. Nakayama;S. Kokubun;H. Kondo
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文献类型：
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作者：
Shigetsune Matsuya;H. Sakagami;A. Tohgo;Y. Owada;Hye-Won Shin;H. Takeshima;K. Nakayama;S. Kokubun;H. Kondo
松谷S.、坂上H.、東郷A.、大和田Y.、申H.W.、竹島H.、中山K.、国分S. 以及近藤H.（2005年）。EFA6家族第三个成员EFA6C在成年小鼠浦肯野细胞中的细胞及亚细胞定位。《神经化学杂志》，93卷，674 - 685页。图4 带有FLAG标签的野生型及GEF缺陷型EFA6C过表达对HeLa细胞肌动蛋白细胞骨架及细胞形态的影响。用编码FLAG - EFA6C或FLAG - EFA6C的GEF缺陷型突变体的表达载体转染HeLa细胞，并用抗FLAG抗体（绿色）、用于F - 肌动蛋白的Alexa568 - 鬼笔环肽（红色）以及用于细胞核的DAPI（蓝色）进行染色。请注意，野生型FLAG - EFA6C的过表达而非GEF缺陷型突变体（E374K）诱导了F - 肌动蛋白聚集的膜突出（箭头所示）。还要注意，GEF缺陷型突变体（E374K）的过表达诱导出许多刺状结构（箭头所示）。比例尺 = 10微米。图3 HeLa细胞中FLAG - EFA6C与ARF1 - HA或ARF6 - HA定位的比较。用编码FLAG - EFA6C和ARF1 - HA或ARF6 - HA的表达载体转染HeLa细胞，并用抗FLAG抗体（红色）和抗HA抗体（绿色）进行免疫染色。用DAPI（蓝色）使细胞核可视化。请注意，在质膜上，FLAG - EFA6C与ARF6 - HA共定位，但不与ARF1 - HA共定位。比例尺 = 10微米。《神经化学杂志》，2005年，95卷，1215页 doi:10.1111/j.1471 - 4159.2005.03421.x
Matsuya S., SakagamiH., Tohgo, A.OwadaY., ShinH.W., TakeshimaH., Nakayama K., Kokubun S. and Kondo H. (2005) Cellular and subcellular localization of EFA6C, a third member of the EFA6 family, in adult mouse Purkinje cells. J. Neurochem. 93, 674–685. Fig. 4 The effect of overexpression of FLAG-tagged wild and GEFdefective EFA6C on actin cytoskeleton and cell morphology of HeLa cells. HeLa cells were transfected with he expression vectors encoding FLAG-EFA6C or GEF-defective mutant of FLAG-EFA6C and stained with anti-FLAG (green), Alexa568-phalloidin (red) for F-actin, and DAPI (blue) for nuclei. Note that the overexpression of wild FLAGEFA6C but not GEF-defective mutant (E374K) induced membrane protrusions where F-actin was accumulated (arrowheads). Also note that the overexpression of GEF-defective mutant (E374K) induced numerous spikes (arrows). Scale bars 1/4 10 lm. Fig. 3 Comparison of localization of FLAG-EFA6C and ARF1-HA or ARF6-HA in HeLa cells. HeLa cells were transfected with the expression vectors encoding FLAG-EFA6C and ARF1-HA or ARF6HA and immunostained with anti-FLAG (red) and anti-HA antibody (green). Nuclei were visualized with DAPI (blue). Note that FLAGEFA6C was colocalized with ARF6-HA but not with ARF1-HA at the plasma membranes. Scale bars 1/4 10 lm. Journal of Neurochemistry, 2005, 95, 1215 doi:10.1111/j.1471-4159.2005.03421.x