LOCALIZED DEPOSITION OF CHITIN ON THE YEAST-CELL SURFACE IN RESPONSE TO MATING PHEROMONE
LOCALIZED DEPOSITION OF CHITIN ON THE YEAST-CELL SURFACE IN RESPONSE TO MATING PHEROMONE
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DOI：
10.1073/pnas.76.2.645
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发表时间：
1979-01-01
影响因子：
11.1
通讯作者：
BRAWLEY, V
中科院分区：
文献类型：
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作者：
SCHEKMAN, R;BRAWLEY, V
用信息素α-处理交配型酿酒酵母细胞因子（由α分泌）交配型细胞）诱导几丁质的合成。开始时没有可检测到的几丁质的小子细胞，当在α-几丁质存在下生长时，产生3倍多的几丁质。因素比未经处理的指数生长细胞更重要。用荧光染料樱草素染色，发现出芽细胞在新生分裂隔膜中积累几丁质。在没有分裂隔膜的情况下，α-因子处理的细胞在信息素刺激的生长区域中积累几丁质。出芽和信息素处理的细胞的酶解允许分离膜结合的几丁质合酶（UDP-2-乙酰氨基-2-脱氧-D-葡萄糖：几丁质4-β-葡聚糖）。乙酰氨基脱氧葡糖基转移酶，EC 2.4.1.16）活性。几丁质合成酶活性与质膜和小的细胞内颗粒两者相关。在信息素处理期间，几丁质合成酶在质膜和细胞内颗粒组分中的水平分别增加11倍和4倍。虽然几丁质合成酶是作为酶原，需要蛋白水解激活，信息素处理的细胞的质膜显示了一个显着部分的预活化酶;细胞内膜结合的合成酶被发现完全在酶原的形式。
Treatment of a mating-type Saccharomyces cerevisiae cells with the pheromone .alpha.-factor (secreted by .alpha. mating-type cells) induces the synthesis of chitin. Small daughter cells, which start with no detectable chitin, make 3 times more chitin when grown in the presence of .alpha.-factor than do untreated exponentially growing cells. Budding cells accumulate chitin in the nascent division septum as detected by staining with the fluorescent dye primulin. In the absence of a division septum, .alpha.-factor-treated cells accumulate chitin in the area of pheromone-stimulated growth. Enzymatic lysis of budding and pheromone-treated cells allows the separation of membrane-bound chitin synthase (UDP-2-acetamido-2-deoxy-D-glucose: chitin 4-.beta.-acetamidodeoxyglucosyltransferase, EC 2.4.1.16) activity from a dense particulate fraction containing chitin. Chitin synthase activity is associated with both the plasma membrane and small intracellular particles. During pheromone treatment, the levels of chitin synthase in the plasma membrane and in intracellular particle fractions increase 11- and 4-fold, respectively. Although chitin synthase is made as zymogen that requires proteolytic activation, the plasma membrane of pheromone-treated cells shows a significant fraction of preactivated enzyme; intracellular membrane-bound synthase is found exclusively in the zymogen form.