Coordinated cell-shape changes control epithelial movement in zebrafish and Drosophila
Coordinated cell-shape changes control epithelial movement in zebrafish and Drosophila
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DOI：
10.1242/dev.02439
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发表时间：
2006-07-15
期刊：
影响因子：
4.6
通讯作者：
Heisenberg, Carl-Philipp
中科院分区：
文献类型：
--
作者：
Koeppen, Mathias;Garcia Fernandez, Beatriz;Heisenberg, Carl-Philipp
上皮的形态发生依赖于细胞形状的协调变化，这一过程仍然知之甚少。在斑马鱼胚珠和果蝇背部闭合过程中，上皮边缘的细胞形状变化是至关重要的。这为这些事件中局部肌动蛋白和肌球蛋白2募集的保守机制提供了证据。研究发现，斑马鱼胚胎外胚外胚层(包膜层)在卵黄细胞表面的运动涉及边缘细胞的收缩。这一过程依赖于肌动蛋白和肌球蛋白2沿被膜层边缘在卵黄细胞质中的募集。肌动蛋白和肌球蛋白2在卵黄细胞质中的重新募集需要Ste20样激酶MSN1，这是果蝇畸形的同源基因。同样，在果蝇中，肌动蛋白和肌球蛋白2在表皮边缘的定位和细胞收缩中介背部闭合，并受畸形控制。因此，这项研究描述了上皮形态发生过程中协调的细胞形状变化背后的保守机制。
Epithelial morphogenesis depends on coordinated changes in cell shape, a process that is still poorly understood. During zebrafish epiboly and Drosophila dorsal closure, cell-shape changes at the epithelial margin are of critical importance. Here evidence is provided for a conserved mechanism of local actin and myosin 2 recruitment during theses events. It was found that during epiboly of the zebrafish embryo, the movement of the outer epithelium (enveloping layer) over the yolk cell surface involves the constriction of marginal cells. This process depends on the recruitment of actin and myosin 2 within the yolk cytoplasm along the margin of the enveloping layer. Actin and myosin 2 recruitment within the yolk cytoplasm requires the Ste20-like kinase Msn1, an orthologue of Drosophila Misshapen. Similarly, in Drosophila, actin and myosin 2 localization and cell constriction at the margin of the epidermis mediate dorsal closure and are controlled by Misshapen. Thus, this study has characterized a conserved mechanism underlying coordinated cell-shape changes during epithelial morphogenesis.