Identification of novel α-synuclein isoforms in human brain tissue by using an online nanoLC-ESI-FTICR-MS method.
Identification of novel α-synuclein isoforms in human brain tissue by using an online nanoLC-ESI-FTICR-MS method.
复制标题
通过使用在线纳米型-si-ftiCR-MS方法，鉴定人脑组织中新型α-突触核蛋白同工型。
DOI：
10.1007/s11064-011-0527-x
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发表时间：
2011-11
影响因子：
4.4
通讯作者：
Westman-Brinkmalm, Ann
中科院分区：
文献类型：
--
作者：
Ohrfelt, Annika;Zetterberg, Henrik;Andersson, Kerstin;Persson, Rita;Secic, Dzemila;Brinkmalm, Gunnar;Wallin, Anders;Mulugeta, Ezra;Francis, Paul T.;Vanmechelen, Eugeen;Aarsland, Dag;Ballard, Clive;Blennow, Kaj;Westman-Brinkmalm, Ann
帕金森病（PD）和路易体痴呆（DLB）是以不同脑区域中路易体的神经元内包涵体为特征的神经退行性疾病。这些包涵体主要由聚集的α-synuclein（α-syn）蛋白组成。本研究采用免疫沉淀结合纳流液相色谱（LC）耦合高分辨率电喷雾离子化傅立叶变换离子回旋共振串联质谱（ESI-FTICR-MS/MS）测定脑组织匀浆中已知和新的α-syn亚型。N-末端乙酰化全长α-syn（Ac-α-syn 1 -140）和两种N-末端乙酰化C-末端截短形式的α-syn结果表明，在正常大鼠颞叶Brodmann 36区（BA 36）和背外侧前额叶BA 9区脑组织匀浆中，α-syn以不同形式存在，DLB和PD伴痴呆（PDD）患者。使用新型酶联免疫吸附试验（ELISA）对每个脑组织部分中的α-syn进行定量。
Parkinson’s disease (PD) and Dementia with Lewy bodies (DLB) are neurodegenerative diseases that are characterized by intra-neuronal inclusions of Lewy bodies in distinct brain regions. These inclusions consist mainly of aggregated α-synuclein (α-syn) protein. The present study used immunoprecipitation combined with nanoflow liquid chromatography (LC) coupled to high resolution electrospray ionization Fourier transform ion cyclotron resonance tandem mass spectrometry (ESI-FTICR-MS/MS) to determine known and novel isoforms of α-syn in brain tissue homogenates. N-terminally acetylated full-length α-syn (Ac-α-syn1–140) and two N-terminally acetylated C-terminally truncated forms of α-syn (Ac-α-syn1–139 and Ac-α-syn1–103) were found. The different forms of α-syn were further studied by Western blotting in brain tissue homogenates from the temporal cortex Brodmann area 36 (BA36) and the dorsolateral prefrontal cortex BA9 derived from controls, patients with DLB and PD with dementia (PDD). Quantification of α-syn in each brain tissue fraction was performed using a novel enzyme-linked immunosorbent assay (ELISA).
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影响因子：
4.7
作者：
Campbell, BCV;McLean, CA;Li, QX
通讯作者：
Li, QX
影响因子：
4.4
作者：
CAMPION, D;MARTIN, C;FREBOURG, T
通讯作者：
FREBOURG, T
影响因子：
9.9
作者：
Ballard, C.;Ziabreva, I.;Aarsland, D.
通讯作者：
Aarsland, D.
影响因子：
14.5
作者：
Hong, Zhen;Shi, Min;Zhang, Jing
通讯作者：
Zhang, Jing
影响因子：
4.8
作者：
Kubo, S;Nemani, VM;Fortin, DL
通讯作者：
Fortin, DL