Infection of human hepatocyte chimeric mouse with genetically engineered hepatitis C virus and its susceptibility to interferon
Infection of human hepatocyte chimeric mouse with genetically engineered hepatitis C virus and its susceptibility to interferon
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DOI：
10.1016/j.febslet.2007.04.021
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发表时间：
2007-05-15
期刊：
影响因子：
3.5
通讯作者：
Chayama, Kazuaki
中科院分区：
文献类型：
--
作者：
Hiraga, Nobuhiko;Imamura, Michio;Chayama, Kazuaki
我们使用感染性克隆和人肝细胞嵌合小鼠建立了丙型肝炎病毒（HCV）基因型Ia和2a的反向遗传学系统。我们静脉接种细胞培养产生的基因型2a（JFH-1）HCV。我们还肝内注射基因型1a CV-H77 C克隆RNA。通过这两种方法接种HCV的小鼠均出现可测量的和可传播的病毒血症。干扰素（IFN）α治疗导致基因型2a HCV水平比基因型Ia更大的降低，如在临床实践中所见。基因工程HCV感染系统的建立将有助于研究HCV对干扰素等药物的耐药机制。(C)2007年欧洲生物化学学会联合会。Elsevier B. V.出版，保留所有权利。
We developed a reverse genetics system of hepatitis C virus (HCV) genotypes la and 2a using infectious clones and human hepatocyte chimeric mice. We inoculated cell culture-produced genotype 2a (JFH-1) HCV intravenously. We also injected genotype 1a CV-H77C clone RNA intrahepatically. Mice inoculated with HCV by both procedures developed measurable and transmissible viremia. Interferon (IFN) alpha treatment resulted in greater reduction of genotype 2a HCV levels than genotype la, as seen in clinical practice. Genetically engineered HCV infection system should be useful for analysis of the mechanisms of resistance of HCV to IFN and other drugs. (C) 2007 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.