Colonic fermentation may play a role in lactose intolerance in humans
Colonic fermentation may play a role in lactose intolerance in humans
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DOI：
10.1093/jn/136.1.58
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发表时间：
2006-01-01
影响因子：
4.2
通讯作者：
Vonk, RJ
中科院分区：
文献类型：
--
作者：
He, T;Priebe, MG;Vonk, RJ
我们之前的研究结果表明，除了小肠乳糖酶活性和运输时间外，结肠对乳糖的加工可能在乳糖不耐症中起作用。我们研究了乳糖的结肠发酵是否与乳糖不耐症有关。28名中国受试者接受1次葡萄糖（安慰剂）和2次乳糖挑战后，根据6小时症状评分对葡萄糖给药后无症状的持续乳糖耐受（n = 7）和不耐受（n = 5）受试者进行分类。在挑战之前，收集粪便样本与乳糖进行体外孵育。在静态系统中厌氧条件下孵育5小时，在此期间取样品测量短链脂肪酸、乳酸、乳糖、葡萄糖和半乳糖。采用荧光原位杂交法测定粪便细菌组成。耐药组和不耐药组在乳糖水解的速率和程度以及葡萄糖和半乳糖的产生方面没有差异。不耐受组产生D-和l -乳酸、乙酸、丙酸和丁酸的速度明显快于耐受组。在不耐受组中，乙酸、丙酸、丁酸和l -乳酸的产生量高于耐受组。两组间粪便细菌组成无差异。结果表明，乳糖水解在结肠中的程度和速率与乳糖不耐症无关。然而，乳糖水解后，微生物中间和末端代谢物更快、更高的产生可能与症状的发生有关。
The results of our previous study suggested that in addition to the small intestinal lactase activity and transit time, colonic processing of lactose may play a role in lactose intolerance. We investigated whether colonic fermentation of lactose is correlated with lactose intolerance. After 28 Chinese subjects had undergone 1 glucose (placebo) and 2 lactose challenges, consistent lactose tolerant (n = 7) and intolerant (n 5) subjects with no complaints after glucose administration were classified on the basis of the 6-h symptom scores. Before the challenges, fecal samples were collected for in vitro incubation with lactose. The incubation was carried out in a static system under anaerobic conditions for 5 h during which samples were taken for measurement of short-chain fatty acids, lactate, lactose, glucose, and galactose. Fecal bacterial composition was determined by fluorescent in situ hybridization. The tolerant and intolerant groups did not differ in the rate or degree of hydrolysis of lactose or production of glucose and galactose. The intolerant group produced D- and L-lactate, acetate, propionate, and butyrate significantly faster than the tolerant group. In the intolerant group, the amounts of acetate, propionate, butyrate, and L-lactate produced were higher than those in the tolerant group. Fecal bacterial composition did not differ between the 2 groups. The results indicate that the degree and rate of lactose hydrolysis in the colon do not play a role in lactose intolerance. However, after lactose is hydrolyzed, a faster and higher production of microbial intermediate and end metabolites may be related to the occurrence of symptoms.