Microtubules are an intracellular target of the plant terpene citral
Microtubules are an intracellular target of the plant terpene citral
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DOI：
10.1111/j.1365-313x.2009.04063.x
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发表时间：
2010-02-01
期刊：
影响因子：
7.2
通讯作者：
Sadot, Einat
中科院分区：
文献类型：
--
作者：
Chaimovitsh, David;Abu-Abied, Mohamad;Sadot, Einat
柠檬醛是植物精油的一种成分，具有多种生物活性。它具有已知的药用特性，并被用作食品添加剂和化妆品。柠檬醛被认为在杂草管理中具有潜力，但其在细胞水平上的确切作用模式尚不清楚。在这里，我们研究了植物细胞的柠檬醛在微摩尔浓度的即时反应。结果发现，拟南芥幼苗微管在暴露于气相柠檬醛后几分钟内被破坏，而肌动蛋白丝保持完整。柠檬醛对植物微管的影响是时间和剂量依赖性的，只有在短暂暴露于柠檬醛几分钟后才能恢复。柠檬醛也能够破坏动物微管，尽管效率较低。此外，在柠檬醛的存在下，微管在体外的聚合被抑制。总之，我们的研究结果表明，柠檬醛是一种有效的，挥发性的，抗微管化合物。
Citral is a component of plant essential oils that possesses several biological activities. It has known medicinal traits, and is used as a food additive and in cosmetics. Citral has been suggested to have potential in weed management, but its precise mode of action at the cellular level is unknown. Here we investigated the immediate response of plant cells to citral at micromolar concentrations. It was found that microtubules of Arabidopsis seedlings were disrupted within minutes after exposure to citral in the gaseous phase, whereas actin filaments remained intact. The effect of citral on plant microtubules was both time- and dose-dependent, and recovery only occurred many hours after a short exposure of several minutes to citral. Citral was also able to disrupt animal microtubules, albeit less efficiently. In addition, polymerization of microtubules in vitro was inhibited in the presence of citral. Taken together, our results suggest that citral is a potent, volatile, anti-microtubule compound.