Electro-acupuncture promotes survival, differentiation of the bone marrow mesenchymal stem cells as well as functional recovery in the spinal cord-transected rats
Electro-acupuncture promotes survival, differentiation of the bone marrow mesenchymal stem cells as well as functional recovery in the spinal cord-transected rats
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DOI：
10.1186/1471-2202-10-35
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发表时间：
2009-04-20
期刊：
影响因子：
2.4
通讯作者：
Zeng, Yuan-Shan
中科院分区：
文献类型：
--
作者：
Ding, Ying;Yan, Qing;Zeng, Yuan-Shan
背景：骨髓间充质干细胞（MSC）是治疗脊髓损伤的潜在工具之一；然而，受损脊髓中间充质干细胞的存活和分化仍需提高。在本研究中，我们探讨督脉电针（EA）能否有效促进横断脊髓中的骨髓间充质干细胞（MSC）的存活和分化、轴突再生以及最终的功能恢复。结果：成年SD大鼠的脊髓在T10时完全横断，分为五个实验组： 1. 假手术对照组 （假手术对照）； 2.操作控制（Op-control）； 3.电针治疗（EA）； 4. 间充质干细胞移植（MSCs）； 5.间充质干细胞移植联合电针（间充质干细胞+电针）。经过2-8周的MSCs移植加EA治疗后，我们发现MSCs+EA组与MSCs移植或单独EA治疗组相比，损伤脊髓病变部位和附近组织中的神经营养蛋白-3（NT-3）、cAMP水平、MSCs的分化、5-HT阳性和CGRP阳性神经纤维显着增加。此外，行为测试和脊髓诱发电位检测显示MSCs+EA组的功能明显恢复。结论：这些结果表明EA治疗可以促进移植的MSCs的存活和分化；间充质干细胞移植联合电针治疗可促进大鼠横断脊髓的轴突再生和部分运动功能恢复，为治疗脊髓损伤提供了一条有希望的途径。
Background: Bone marrow mesenchymal stem cells (MSCs) are one of the potential tools for treatment of the spinal cord injury; however, the survival and differentiation of MSCs in an injured spinal cord still need to be improved. In the present study, we investigated whether Governor Vessel electro-acupuncture (EA) could efficiently promote bone marrow mesenchymal stem cells (MSCs) survival and differentiation, axonal regeneration and finally, functional recovery in the transected spinal cord.Results: The spinal cords of adult Sprague-Dawley (SD) rats were completely transected at T10, five experimental groups were performed: 1. sham operated control (Sham-control); 2. operated control (Op-control); 3. electro-acupuncture treatment (EA); 4. MSCs transplantation (MSCs); and 5. MSCs transplantation combined with electro-acupuncture (MSCs+EA). After 2-8 weeks of MSCs transplantation plus EA treatment, we found that the neurotrophin-3 (NT-3), cAMP level, the differentiation of MSCs, the 5-HT positive and CGRP positive nerve fibers in the lesion site and nearby tissue of injured spinal cord were significantly increased in the MSCs+EA group as compared to the group of the MSCs transplantation or the EA treated alone. Furthermore, behavioral test and spinal cord evoked potentials detection demonstrated a significantly functional recovery in the MSCs+EA group.Conclusion: These results suggest that EA treatment may promote grafted MSCs survival and differentiation; MSCs transplantation combined with EA treatment could promote axonal regeneration and partial locomotor functional recovery in the transected spinal cord in rats and indicate a promising avenue of treatment of spinal cord injury.