RAPD Analysis of Genetic Variation Within and Among Four Natural Populations of Betula maximowicziana
RAPD Analysis of Genetic Variation Within and Among Four Natural Populations of Betula maximowicziana
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DOI：
10.1515/sg-2004-0042
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发表时间：
2004-12
期刊：
影响因子：
1
通讯作者：
Y. Tsuda;S. Goto;Y. Ide
中科院分区：
文献类型：
--
作者：
Y. Tsuda;S. Goto;Y. Ide
西南桦（Betula maximowicziana）是冷温带森林中的长寿先锋树种，在森林生态系统中具有重要作用，具有较高的经济价值。利用随机扩增多态性DNA（RAPD）标记对B 4个自然居群的遗传变异进行了分析。maximowicziana（三个在本州中部，另一个在北海道），以获得关于这种自然资源的基本信息。23个引物共扩增出61条扩增带。在这61条谱带中，22条为单态谱带，39条为多态谱带。每个种群内的遗传变异水平可能非常相似，因为根据多态位点数、香农指数和种群内遗传方差进行的种群排名没有显示出明确的模式。AMOVA分析表明，居群间遗传变异占总变异的15.6%，居群内遗传变异占84.4%。这些结果表明，四个群体之间的遗传分化是适度的。层次AMOVA分析表明，区域间（北海道和本州中部）的变异占总遗传变异的10.4%，表明区域遗传分化相对较高。两两ΦPT值和地理距离之间的显着相关性被检测到，和两个相邻连接的聚类图的基础上两ΦPT值的结果，和主坐标分析（PCO）的基础上的欧几里德度量显示，富良野居群在北海道从中部本州的3个群体的遗传差异。本研究获得的数据对B的区域遗传变异的保护和管理具有重要意义。maximowicziana。
Summary Betula maximowicziana is a long-lived pioneer tree species in cool temperate forests that plays an important role in the forest ecosystem and has high economic value. Random amplified polymorphic DNA (RAPD) markers were used to evaluate the genetic variation of four natural populations of B. maximowicziana (three in central Honshu and the other in Hokkaido) to obtain fundamental information on this natural resource. Sixty-one reproductive amplified bands were obtained with 23 primers. Of these 61 bands, 22 were monomorphic and 39 were polymorphic. The level of genetic variation within each population may be very similar, because the population rankings according to number of polymorphic loci, Shannon’s indices and intra-population genetic variance revealed no definite patterns. Global analysis of AMOVA (analysis of molecular variance) showed that genetic variation among populations accounted for 15.6% of the total variation, with the remainder (84.4%) occurring within population. These results demonstrate that genetic differentiation among the four populations is moderate. Hierarchical AMOVA analysis showed that variation among regions (Hokkaido and central Honshu) accounted for 10.4% of the total genetic variation, suggesting that regional genetic differentiation is relatively high. Significant correlations between pairwise ΦPT values and geographic distance were detected, and results of both a neighbor-joining dendrogram based on pairwise ΦPT values, and principal coordinate analysis (PCO) based on a Euclidean metric revealed that the Furano population in Hokkaido was genetically different from the three populations in central Honshu. The data obtained in this study should have important implications for the conservation and management of regional genetic variation of B. maximowicziana.