Fas/APO-1 (CD95) expression in myelodysplastic syndromes.
Fas/APO-1 (CD95) expression in myelodysplastic syndromes.
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Fas/APO-1 (CD95) 在骨髓增生异常综合征中的表达。
DOI：
10.3109/10428199809057543
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发表时间：
1998
影响因子：
2.6
通讯作者：
P. Fenaux
中科院分区：
文献类型：
--
作者：
P. Lepelley;N. Grardel;O. Erny;T. Iaru;V. Obein;A. Cosson;P. Fenaux
骨髓增生异常综合征（MDS）中细胞减少的病理生理机制似乎与髓系前体细胞凋亡增加有关。Fas /APO-1（CD95）是一种与Fas配体或功能性抗Fas抗体结合后诱导凋亡信号的细胞表面蛋白。本研究用免疫细胞化学方法研究了30例MDS骨髓载玻片上Fas的表达。与对照组相比，在12例（40%）病例中，红细胞和/或未成熟粒细胞中Fas表达增加。此外，在18例>或= 5%的骨髓母细胞中，有16例表达Fas的母细胞比例不同。形态学分析和/或TUNEL技术发现26例患者中8例（31%）骨髓细胞凋亡增加。在5例Fas过表达患者中加入功能性抗Fas抗体后，研究Fas抗原触发细胞凋亡的能力。然而，该抗体的加入只导致5例中2例未成熟粒细胞（而非其他髓系细胞）的凋亡轻微增加。因此，在大多数MDS病例中，骨髓和/或母细胞中Fas表达增加。然而，这一发现与MDS细胞凋亡增加之间的关系仍有待确定。
Increased apoptosis of myeloid precursors appears to contribute to the pathophysiology of cytopenias in myelodysplastic syndromes (MDS). Fas /APO-1(CD95) is a cell surface protein inducing an apoptotic signal after its binding to Fas ligand or to a functional anti-Fas antibody. Here we studied Fas expression by immunocytochemistry on marrow slides from 30 cases of MDS. Increased Fas expression in erythroblasts and/or immature granulocytes, compared to controls, was seen in 12 (40%) of the cases. In addition, in 16 of the 18 cases with > or = 5% marrow blasts, a variable proportion of blasts expressed Fas. Increased apoptosis was found by morphological analysis and/or TUNEL technique in marrow cells from 8 of the 26 cases analyzed (31%) The ability of Fas antigen to trigger apoptosis was studied after addition of a functional anti Fas antibody in 5 of the patients with Fas overexpression. Addition of this antibody, however, only lead to mild increase of apoptosis in immature granulocytes (but not other myeloid cells) in 2 of the 5 cases. Thus, increased Fas expression is seen in myeloid and/or blast cells in the majority of MDS cases. However, the relationship between this finding and increased apoptosis in MDS still remains to be established.
影响因子：
20.3
作者：
RAZA, A;GEZER, S;PREISLER, H
通讯作者：
PREISLER, H