Synaptonemal complex protein SYCP3: Conserved polymerization properties among vertebrates
Synaptonemal complex protein SYCP3: Conserved polymerization properties among vertebrates
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DOI：
10.1016/j.bbapap.2007.03.008
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发表时间：
2007-05-01
影响因子：
3.2
通讯作者：
Benavente, Ricardo
中科院分区：
文献类型：
--
作者：
Baier, Andrea;Alsheimer, Manfred;Benavente, Ricardo
联会复合体（SC）是减数分裂特异性的核结构，其在同源染色体的联会、重组和分离中起重要作用。虽然SC结构在进化中是保守的，但其蛋白质组分并非如此。为了提供SC蛋白的信息，这将是重要的，我们了解保守的SC结构和功能，在这里，我们比较了同源SYCP3蛋白的两个进化遥远的脊椎动物物种，即大鼠和青鳉鱼。为此，我们已经调查了这两种蛋白质的聚合特性，免疫细胞化学，电子显微镜和细胞分级。我们发现，尽管在过去的4.5亿年中积累了序列差异，但哺乳动物和鱼类SYCP3具有相似的特性，使它们能够在实验条件下共同组装更高级的结构。我们还提供了一个可能的解释，如何杂合突变SYCP3基因可以导致有缺陷的减数分裂。(C)2007 Elsevier B.V.保留所有权利。
Synaptonemal complexes (SCs) are meiosis-specific, nuclear structures that are critically involved in synapsis, recombination and segregation of homologous chromosomes. Although the SC structure is conserved in evolution this is not the case for its protein components. To provide information on SC proteins which would be important for our understanding of the conserved SC structure and function, here we compared ortholog SYCP3 proteins of two evolutionary distant vertebrate species, namely rat and medaka fish. To this end we have investigated the polymerization properties of both proteins by immunocytochemistry, electron microscopy and cell fractionation. We found that despite of the sequence differences that have accumulated over the last 450 million years mammalian and fish SYCP3 have similar properties that allow them to co-assemble higher order structures under experimental conditions. We also provide a likely explanation as to how heterozygous mutations in the SYCP3 gene can lead to a defective meiosis. (C) 2007 Elsevier B.V. All rights reserved.