MARBURG VIRUS, A FILOVIRUS - MESSENGER-RNAS, GENE ORDER, AND REGULATORY ELEMENTS OF THE REPLICATION CYCLE
MARBURG VIRUS, A FILOVIRUS - MESSENGER-RNAS, GENE ORDER, AND REGULATORY ELEMENTS OF THE REPLICATION CYCLE
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DOI：
10.1016/0168-1702(92)90027-7
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发表时间：
1992-06-01
期刊：
影响因子：
5
通讯作者：
KLENK, HD
中科院分区：
文献类型：
--
作者：
FELDMANN, H;MUHLBERGER, E;KLENK, HD
马尔堡病毒（MBG）是一种丝状病毒，其基因组长度为19.1 kb，因此是在负链RNA病毒中发现的最大基因组。基因顺序- 3'非翻译区-NP-VP 35-VP 40-GP-VP 30-VP 24-L-5'非翻译区-类似于其他非节段负链（NNS）RNA病毒。从MBG感染的细胞中分离的六种多聚腺苷酸化亚基因组RNA与负链RNA基因组互补。它们可以在体外翻译成已知的结构蛋白NP、GP（非糖基化形式）、VP 40、VP 35、VP 30和VP 24。在基因边界处，保守的转录起始信号（3 '-NNCUNCNUNAAUU-5'）和终止信号（3 '-UAAUUCUUUU 5'）位于含有高度保守的五聚体3 '-UAAUU-5'的位置。与其他NNS RNA病毒的比较显示，主要是在终止信号的保守性，而开始信号更多变。基因间区的长度和核苷酸组成不同。所有基因都具有相对长的3'和5'端非编码区。MBG基因组的推定的3'和5'前导RNA序列在长度、3'和5'末端的保守性以及末端的互补性方面与其他NNS RNA病毒相似。数据支持的概念，一个共同的分类单负性病毒目包括丝状病毒科，副粘病毒科，弹状病毒科。
The genome of Marburg virus (MBG), a filovirus, is 19.1 kb in length and thus the largest one found with negative-strand RNA viruses. The gene order - 3' untranslated region-NP-VP35-VP40-GP-VP30-VP24-L-5' untranslated region-resembles that of other non-segmented negative-strand (NNS) RNA viruses. Six species of polyadenylated subgenomic RNAs, isolated from MBG-infected cells, are complementary to the negative-strand RNA genome. They can be translated in vitro into the known structural proteins NP, GP (non-glycosylated form), VP40, VP35, VP30 and VP24. At the gene boundaries conserved transcriptional start (3'-NNCUNCNUNUAAUU-5') and stop signals (3'-UAAUUCUUUUU5') are located containing the highly conserved pentamer 3'-UAAUU-5'. Comparison with other NNS RNA viruses shows conservation primarily in the termination signals, whereas the start signals are more variable. The intergenic regions vary in length and nucleotide composition. All genes have relatively long 3' and 5' end non-coding regions. The putative 3' and 5' leader RNA sequences of the MBG genome resemble those of other NNS RNA viruses in length, conservation at the 3' and 5' ends, and in being complementary at their extremities. The data support the concept of a common taxonomic order Mononegavirales comprising the Filoviridae, Paramyxoviridae, and Rhabdoviridae families.