Prevalence and Sequence-Based Identity of Rumen Fluke in Cattle and Deer in New Caledonia.
Prevalence and Sequence-Based Identity of Rumen Fluke in Cattle and Deer in New Caledonia.
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DOI：
10.1371/journal.pone.0152603
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Zadoks R
中科院分区：
文献类型：
--
作者：
Cauquil L;Hüe T;Hurlin JC;Mitchell G;Searle K;Skuce P;Zadoks R
在新喀里多尼亚的法属美拉尼西亚群岛进行了一项屠宰场调查，以确定牛和鹿中副胃动物的流行情况，并为在种和亚种水平上进行分子分型提供材料。在动物水平（387头成年牛中占70%）和批次水平（81%）上，成年牛的患病率较高。在两个水平上，犊牛的患病率都较低（484头犊牛中有33%，批次水平上有51%）。7个鹿场中有2个动物瘤胃吸虫阳性，动物水平感染率分别为41.4%（29/70）和47.1%（33/70）。利用ITS-2测序技术，鉴定出3种副端虫，分别为Calicophoron calicophorum、Fischoederius elongatus和Orthocoelium streptocoelium。在牛和鹿中都检测到这三种病毒，这表明瘤胃吸虫可能在两种宿主之间传播。基于ITS-2序列的异质性，C. calicophorum种群包括两个分支，这两个分支都存在于牛和鹿中。结果表明，瘤胃吸虫有两条不同的传入途径。这种方法在调查瘤胃吸虫感染的起源和牲畜-野生动物界面上寄生虫传播的可能性方面具有更广泛的适用性。
An abattoir survey was performed in the French Melanesian archipelago of New Caledonia to determine the prevalence of paramphistomes in cattle and deer and to generate material for molecular typing at species and subspecies level. Prevalence in adult cattle was high at animal level (70% of 387 adult cattle) and batch level (81%). Prevalence was lower in calves at both levels (33% of 484 calves, 51% at batch level). Animals from 2 of 7 deer farms were positive for rumen fluke, with animal-level prevalence of 41.4% (29/70) and 47.1% (33/70), respectively. Using ITS-2 sequencing, 3 species of paramphistomes were identified, i.e. Calicophoron calicophorum, Fischoederius elongatus and Orthocoelium streptocoelium. All three species were detected in cattle as well as deer, suggesting the possibility of rumen fluke transmission between the two host species. Based on heterogeneity in ITS-2 sequences, the C. calicophorum population comprises two clades, both of which occur in cattle as well as deer. The results suggest two distinct routes of rumen fluke introduction into this area. This approach has wider applicability for investigations of the origin of rumen fluke infections and for the possibility of parasite transmission at the livestock-wildlife interface.