Characterization of Toxoplasma gondii isolates in free-range chickens from Chile, South America
Characterization of Toxoplasma gondii isolates in free-range chickens from Chile, South America
复制标题
DOI：
10.1016/j.vetpar.2006.03.023
复制
发表时间：
2006-08-31
影响因子：
2.6
通讯作者：
Shen, S. K.
中科院分区：
文献类型：
--
作者：
Dubey, J. P.;Patitucci, A. N.;Shen, S. K.
由于鸡是从地面取食的，因此，散养鸡中弓形虫的流行是土壤中弓形虫卵囊流行的一个很好的指标。本文对85只来自智利的鸡进行了弓形虫感染率的测定。用改良凝集试验（MAT）检测了85只鸡中47只（55.3.9%）的弓形虫抗体，其滴度为1：5的6只，1：10的4只，1：20的4只，1：40的3只，1：80的9只，1：160的4只，1：320的9只，1：640以上的8只。将滴度为1：5或更高的47只鸡的心脏和脑合并用于每只鸡并在小鼠中进行生物测定。将来自16只血清阴性（MAT < 1：5）的鸡的组织合并并饲喂给一只无刚地弓形虫的猫。检查猫的粪便中的卵囊，但基于小鼠粪便漂浮物的生物测定，未发现卵囊。将来自7只血清阴性（< 1：5）的心脏和大脑合并，并在小鼠中进行生物测定;未分离出弓形虫。用生物测定法从22只鸡体内分离出弓形虫，其MAT滴度为1：20或更高。使用SAG 1、SAG 2、SAG 3、BTUB和GRA 6位点的多态性对这22个分离株进行基因分型，发现了三种基因型。17个菌株在所有位点上具有II型等位基因，4个菌株具有III型等位基因。一个分离物含有I型和III型等位基因的组合。这是首次报道南美洲智利弓形虫分离株的遗传特征。(c)2006 Elsevier B. V.保留所有权利。
The prevalence of Toxoplasma gondii in free-ranging chickens is a good indicator of the prevalence of T gondii oocysts in the soil because chickens feed from the ground. The prevalence of T gondii in 85 free-range chickens (Gallus domesticus) from Chile was determined. Antibodies to T gondii were assayed by the modified agglutination test (MAT), and found in 47 of 85 (55.3.9%) chickens with titers of 1:5 in six, 1: 10 in four, 1:20 in four 1: 40 in three, 1: 80 in nine, 1: 160 in four 1:320 in nine, and 1: 640 or higher in eight. Hearts and brains of 47 chickens with titers of 1:5 or higher were pooled for each chicken and bioassayed in mice. Tissues from 16 seronegative (MAT < 1:5) chickens were pooled and fed to one T gondii-free cat. Feces of the cat were examined for oocysts but none was found based on bioassay of fecal floats in mice. Hearts and brains from seven seronegative (< 1:5) were pooled and bioassayed in mice; T gondii was not isolated. T gondii was isolated by bioassay in mice from 22 chickens with MAT titers of 1:20 or higher. Genotyping of these 22 isolates using polymorphisms at the loci SAG1, SAG2, SAG3, BTUB and GRA6 revealed three genotypes. Seventeen isolates had type II alleles and four isolates had type III alleles at all loci. One isolate contained the combination of type I and III alleles. This is the first report of genetic characterization of T gondii isolates from Chile, South America. (c) 2006 Elsevier B.V. All rights reserved.