Effects of esculin and esculetin on the survival of Escherichia coli O157 in human faecal slurries, continuous-flow simulations of the rumen and colon and in calves
Effects of esculin and esculetin on the survival of Escherichia coli O157 in human faecal slurries, continuous-flow simulations of the rumen and colon and in calves
复制标题
DOI：
10.1079/bjn20041101
复制
发表时间：
2004-05-01
影响因子：
3.6
通讯作者：
Stewart, CS
中科院分区：
文献类型：
--
作者：
Duncan, SH;Leitch, ECM;Stewart, CS
人类病原体O157：H7被认为是通过直接或间接接触受感染的动物或人的粪便而传播的。本文研究了植物香豆素、七叶皂素及其苷元秦皮乙素对肠道条件下一株大肠杆菌O157存活的影响。将这些化合物添加到人类粪便浆液和模拟人类结肠和瘤胃条件的体外连续流动发酵模型中，导致一种引入的大肠杆菌O157菌株的存活率显著下降。当4头犊牛实验感染O157大肠杆菌并饲喂七叶皂苷时，接种后检查的28份粪便样本中有5份(18%)检出了病原体，而没有饲喂秦皮甲素的5头对照犊牛的35份粪便样本中有13份(37%)检出了病原体。天然存在于食用植物中或在饮食中添加的香豆素化合物可能会抑制大肠杆菌O157在肠道中的存活。
The human pathogen Escherichia coli O157:H7 is thought to be spread by direct or indirect contact with infected animal or human faeces. The present study investigated the effects of the plant coumarin esculin and its aglycone esculetin on the survival of a strain of E. coli O157 under gut conditions. The addition of these compounds to human faecal slurries and in vitro continuous-flow fermenter models simulating conditions in the human colon and rumen caused marked decreases in the survival of an introduced strain of E. coli O157. When four calves were experimentally infected with E. coli O157 and fed esculin, the pathogen was detected in five of twenty-eight (18%) of faecal samples examined post-inoculation, compared with thirteen of thirty-five (37%) of faecal samples examined from five control calves not fed esculin. Coumarin compounds that occur naturally in dietary plants or when supplemented in the diet probably inhibit the survival of E. coli O157 in the gut.