MicroRNA-195-5p suppresses glucose uptake and proliferation of human bladder cancer T24 cells by regulating GLUT3 expression
MicroRNA-195-5p suppresses glucose uptake and proliferation of human bladder cancer T24 cells by regulating GLUT3 expression
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DOI：
10.1016/j.febslet.2012.01.006
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发表时间：
2012-02-17
期刊：
影响因子：
3.5
通讯作者：
Li, Tingting
中科院分区：
文献类型：
--
作者：
Fei, Xiang;Qi, Manlong;Li, Tingting
据报道，葡萄糖转运蛋白3（GLUT 3）的表达在膀胱癌中上调。然而，其调控机制尚不清楚。在这里，我们评估了microRNAs（miRNAs）是否调节膀胱癌中GLUT 3的表达。在我们的研究中，miR-195- 5 p被鉴定为直接靶向膀胱癌T24细胞中的GLUT 3 3 '-非翻译区（UTR）。小干扰RNA（siRNA）和miR-195- 5 p介导的GLUT 3敲低实验表明，miR-195- 5 p通过抑制GLUT 3表达，降低T24细胞葡萄糖摄取，抑制细胞生长，促进细胞凋亡。因此，miR-195- 5 p是一种新的，也是第一个被鉴定的靶向GLUT 3的miRNA，miR-195- 5 p的异常表达降低和随后的GLUT 3上调可能有助于膀胱癌的发生。(C)2012年欧洲生物化学学会联合会。由Elsevier B出版。V.保留所有权利。
It has been reported that expression of glucose transporter member 3 (GLUT3) is up-regulated in bladder cancers. However, the regulating mechanism remains unknown. Here, we assessed whether microRNAs (miRNAs) regulate GLUT3 expression in bladder cancers. In our study, miR-195-5p was identified to directly targeted GLUT3 3'-untranslated region (UTR) in bladder cancer T24 cells. Small interfering RNA (siRNA)- and miR-195-5p-mediated GLUT3 knockdown experiments revealed that miR-195-5p decreased T24 cells glucose uptake, inhibited cell growth and promoted cell apoptosis through suppression of GLUT3 expression. Therefore, miR-195-5p is a novel and also the first identified miRNA that targets GLUT3, and the aberrant decreased expression of miR-195-5p and consequent GLUT3 up-regulation may contribute to bladder carcinogenesis. (C) 2012 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.