Glyco-modification of protein with O-cyanate chain-end functionalized glycopolymer via isourea bond formation.
Glyco-modification of protein with O-cyanate chain-end functionalized glycopolymer via isourea bond formation.
复制标题
通过形成异脲键，用 O-氰酸酯链端功能化的糖聚合物对蛋白质进行糖修饰。
DOI：
10.1080/07328303.2014.922189
复制
发表时间：
2014
影响因子：
1
通讯作者：
Sun,Xue-Long
中科院分区：
文献类型：
--
作者：
Gruzdys,Valentinas;Zhang,Hailong;Sun,Xue-Long
Glycoengineering aimed at the addition of carbohydrates to proteins is an attractive approach to alter the pharmacokinetic properties of proteins, such as enhancing stability and prolonging the duration of action. We report a novel protein glyco-modification of BSA and recombinant thrombomodulin withO-cyanate chain-end functionalized glycopolymer via isourea bond formation. The protein glycoconjugates were confirmed by SDS-PAGE, western blot, and MALDI-TOF mass spectrometry. Protein C activation activity of the glyco-modified recombinant thrombomodulin was confirmed, proving no interference with activity from the glycopolymer modification. The isourea bond formation under mild conditions was demonstrated as an alternative method for protein modification with polymers.
