Effects of Polysaccharides from Dendrobium Officinale on Intestinal Barrier Functions of Rats Induced by Excessive Consumption of High Fat and Sucrose Diet
Effects of Polysaccharides from Dendrobium Officinale on Intestinal Barrier Functions of Rats Induced by Excessive Consumption of High Fat and Sucrose Diet
复制标题
铁皮石斛多糖对高脂高糖饮食大鼠肠道屏障功能的影响
DOI：
10.1096/fasebj.2019.33.1_supplement.516.9
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发表时间：
2019-04
期刊：
影响因子：
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通讯作者：
Guiyuan Lv
中科院分区：
文献类型：
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作者：
Meiqiu Yan;Qiuqiu Si;Jie Su;Jinjin Yu;Shanshan Lei;Bo Li;Ting Wang;Zhiyuan Yang;Suhong Chen;Guiyuan Lv
越来越多的证据表明，不适当的饮食可以损害宿主的肠道屏障功能，增加血液中的内毒素水平。铁皮石斛作为一种主要用于治疗糖尿病的传统中药，对多种代谢性疾病均有明显的保护作用。同时具有明显的调节肠道菌群失调、改善肠道屏障、促进消化等胃肠功能，多糖已被证实是其主要有效部位。本研究通过连续10周灌胃给药，观察铁皮石斛多糖（DOP）对大鼠肠道屏障功能的影响，包括对机械屏障和生物屏障功能的影响。结果表明，DOP可显著降低大鼠血清TC、GLU和HOMA-IR水平，上调回肠claudin-1、occludin表达，显著提高血清白细胞介素-6（IL-6）和肿瘤坏死因子-α（TNF-α）水平，降低血清二胺氧化酶（DAO）和脂多糖（LPS）水平。同时，多糖降低了厚壁菌与拟杆菌的比例，并上调了乙酸盐、丙酸盐和丁酸盐的产生。结果表明，石斛多糖可能通过调节肠道屏障功能，改善高脂高糖饮食诱导的胰岛素抵抗。
Accumulating evidence indicates that improper diet can damage host gut barrier function and increase endotoxin level in the bloodstream. Dendrobium officinale as a traditional herb medicine mainly used for diabetes showed obvious protective effect in various metabolic diseases. Meanwhile it has obvious enterogastric function, such as modulating of enteric dysbacteriosis, improving gut barrier, promoting digestion, etc. Polysaccharides have been verified are the main effective fraction. In present study, polysaccharides from Dendrobium Officinale(DOP) was given via oral administration to rats for 10 weeks to investigate its effects on intestinal barrier functions, including the mechanical barrier and biological barrier function. It was found that DOP administration could significantly down‐regulate the level of TC, GLU and HOMA‐IR, up‐regulate the expression of claudin‐1, occludin in ileum, markedly improve the levels of interleukin‐6 (IL‐6) and tumor necrosis factor ‐α(TNF‐α), and decrease the level of diamine oxidase (DAO) and lipopolysaccharides (LPS) in serum. Meanwhile, the polysaccharide decreased the Firmicutes‐to‐Bacteroidetes ratio and upregulated the production of acetate, propionate and butyrate. Our results indicated that the polysaccharides from stem of Dendrobium Officinale might ameliorate insulin resistance induced by high fat and sucrose diet by regulate the intestinal barrier functions.