Mass production of human airway-like neutrophils via transmigration in an organotypic model of human airways.
Mass production of human airway-like neutrophils via transmigration in an organotypic model of human airways.
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DOI：
10.1016/j.xpro.2021.100892
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发表时间：
2021-12-17
期刊：
影响因子：
--
通讯作者：
Tirouvanziam R
中科院分区：
文献类型：
--
作者：
Dobosh B;Giacalone VD;Margaroli C;Tirouvanziam R
中性粒细胞很难研究，特别是在组织中，因为它们的半衰期很短，而且容易被激活。为了更好地了解中性粒细胞在呼吸道疾病中的作用，我们描述了一种器官型呼吸道模型，该模型使用患者的呼吸道液体使血液中性粒细胞的转运获得呼吸道样表型。特别是，我们展示了条件中性粒细胞如何调节他们的杀菌能力。有关本议定书的使用和执行的完整细节，请参阅。肺器官型模型允许上皮细胞在气液界面上生长主要的血液中性粒细胞(和其他白细胞)可以跨上皮细胞迁移患者气道液添加顶部条件迁移细胞，这反映了体内状态的药物可用于了解潜在的机制，导致疾病中性粒细胞很难研究，特别是在组织中，由于其短的半衰期和激活的倾向。为了更好地了解中性粒细胞在呼吸道疾病中的作用，我们描述了一种器官型呼吸道模型，该模型使用患者的呼吸道液体使血液中性粒细胞的转运获得呼吸道样表型。特别是，我们展示了条件中性粒细胞如何调节他们的杀菌能力。
Neutrophils are difficult to study, particularly in tissues, due to their short half-life and propensity for activation. We describe an organotypic airway model that uses patient airway fluid to enable the transmigration of blood neutrophils to acquire an airway-like phenotype in order to better understand their contribution to airway diseases. In particular, we showcase how conditioned neutrophils modulate their bacteria-killing abilities. For complete details on the use and execution of this protocol, please refer to. A lung organotypic model allows epithelial cells to be grown at an air-liquid interface Primary blood neutrophils (and other leukocytes) can transmigrate across the epithelium Patient airway fluid added apically conditions transmigrating cells, which mirrors the in vivo state Drugs can be used to understand underlying mechanisms driving disease Neutrophils are difficult to study, particularly in tissues, due to their short half-life and propensity for activation. We describe an organotypic airway model that uses patient airway fluid to enable the transmigration of blood neutrophils to acquire an airway-like phenotype in order to better understand their contribution to airway diseases. In particular, we showcase how conditioned neutrophils modulate their bacteria-killing abilities.
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DOI：
10.1016/j.xcrm.2021.100239
发表时间：
2021-04-20
期刊：
Cell reports. Medicine
影响因子：
--
作者：
Margaroli C;Moncada-Giraldo D;Gulick DA;Dobosh B;Giacalone VD;Forrest OA;Sun F;Gu C;Gaggar A;Kissick H;Wu R;Gibson G;Tirouvanziam R
通讯作者：
Tirouvanziam R
影响因子：
5.6
作者：
Ford BD;Moncada Giraldo D;Margaroli C;Giacalone VD;Brown MR;Peng L;Tirouvanziam R
通讯作者：
Tirouvanziam R
影响因子：
4.6
作者：
Grunwell, Jocelyn R.;Giacalone, Vincent D.;Tirouvanziam, Rabindra
通讯作者：
Tirouvanziam, Rabindra
影响因子：
5.5
作者：
Forrest, Osric A.;Ingersoll, Sarah A.;Tirouvanziam, Rabindra
通讯作者：
Tirouvanziam, Rabindra
影响因子：
24.3
作者：
Loppow, D;Böttcher, M;Jörres, RA
通讯作者：
Jörres, RA