Expression analysis of sex-specific and 17β-estradiol-responsive genes in the Japanese medaka, Oryzias latipes, using oligonucleotide microarrays
Expression analysis of sex-specific and 17β-estradiol-responsive genes in the Japanese medaka, Oryzias latipes, using oligonucleotide microarrays
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DOI：
10.1016/j.ygeno.2006.03.023
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发表时间：
2006-08-01
期刊：
影响因子：
4.4
通讯作者：
Iwahashi, Hitoshi
中科院分区：
文献类型：
--
作者：
Kishi, Katsuyuki;Kitagawa, Emiko;Iwahashi, Hitoshi
利用代表22，587个TIGRO的寡核苷酸DNA微阵列对日本青鳉（Oryzias latipes）进行基因分析。latipes基因指数（OLGI）。个体成熟鱼之间的基因表达的平均相关系数对于雌性和雄性都很高（> 0.95），表明青鳉的生理状态在规定的生长条件下是高度可重复的。在22，587个OLGI中，2575个在女性和男性之间的表达存在显着差异。暴露于17 β-雌二醇（E2）揭示了381 E2响应OLGI在男性青鳉。女性化和男性功能障碍因素的E2处理的男性使用皮尔逊相关系数和欧氏距离的组合计算表明，E2治疗“弱女性化”的男性青鳉，而男性的生理功能没有显着中断。这项研究证明了使用青鳉微阵列来估计生殖活性化学物质的总体影响的可能性。(c)2006年爱思唯尔公司All rights reserved.
Gene profiling of Japanese medaka (Oryzias latipes) was performed using an oligonucleotide DNA microarray representing 22,587 TIGR O. latipes gene indices (OLGIs). The average correlation coefficients for gene expression between individual mature fish were high (> 0.95) for both female and male, indicating that the physiological status of medaka is highly reproducible under prescribed growth conditions. Of the 22,587 OLGIs, 2575 showed significant differences in expression between female and male. Exposure to 17 beta-estradiol (E2) revealed 381 E2-responsive OLGIs in male medaka. Feminization and male-dysfunction factors of the E2-treated males calculated using the combination of Pearson correlation coefficient and Euclidean distances indicate that E2 treatment "weakly feminized" male medaka, while male physiological functions were not significantly disrupted. This study demonstrates the possibility of using medaka microarrays to estimate the overall effects of hormonally active chemicals. (c) 2006 Elsevier Inc. All rights reserved.