Nitric oxide is involved in male sexual behavior of rats
Nitric oxide is involved in male sexual behavior of rats
复制标题
DOI：
10.1016/0014-2999(95)00578-1
复制
发表时间：
1995-12-29
影响因子：
5
通讯作者：
Arletti, R
中科院分区：
文献类型：
--
作者：
Benelli, A;Bertolini, A;Arletti, R
在性经验和性幼稚的雄性大鼠中，腹腔注射L精氨酸(一氧化氮合酶的天然底物)(10，25，50 mg/kg)均可增加性幼稚大鼠的交配百分率，改善性经验大鼠的性行为指数，而腹腔注射一氧化氮合酶的有效抑制剂L精氨酸甲酯(10，25，50 mg/kg)则有相反的作用。而侧脑室注射L精氨酸(25、50、100mgg/只)后，无论是幼龄大鼠还是有经验的大鼠，均无明显作用，而L则完全阻止幼稚大鼠的射精，剂量为100mUg/只，而对有经验的大鼠则完全无效，直至300mg/只。最后，雄性交配能力与大脑中一个离散而明确的区域--下丘脑室旁核--的一氧化氮合酶活性之间似乎存在直接关系：的确，在性能力强的大鼠的这个核团中，一氧化氮合酶的mRNA表达大约是性无能大鼠的两倍。结论是，一氧化氮合酶参与了男性性活动的表达，尽管存在一些难以解释的矛盾。
In male rats, whether sexually experienced or sexually naive, the intraperitoneal administration of L-arginine (the natural substrate for nitric oxide synthase) (10, 25, 50 mg/kg) both increased the percentage of copulating in sexually naive rats and improved the indexes of sexual performance in sexually experienced rats, whereas the intraperitoneal administration of N-G-nitro-L-arginine methyl ester (L-NAME) (a potent inhibitor of nitric oxide synthase) (10, 25, 50 mg/kg) had opposite effects. In contrast, after intracerebroventricular administration, L-arginine (25, 50, 100 mu g/rat) had no effect - whether in naive or in experienced rats - whereas L-NAME completely prevented ejaculation in naive rats, at the dose of 100 mu g/rat, but had no effect at all in experienced rats, up to the dose of 300 mu g/rat. Finally, a direct relationship seems to exist between male copulatory performance and nitric oxide synthase activity in a discrete and defined brain area, the paraventricular nucleus of the hypothalamus: indeed, nitric oxide synthase mRNA expression in this nucleus in sexually potent rats is about twice that in sexually impotent rats. It is concluded that nitric oxide synthase is involved in the expression of male sexual activity, in spite of some inconsistencies that are hard to interpret.