Inhibitory Effect of Coenzyme Q0 on the Growth of Staphylococcus aureus
Inhibitory Effect of Coenzyme Q0 on the Growth of Staphylococcus aureus
复制标题
辅酶Q0对金黄色葡萄球菌生长的抑制作用
DOI：
10.1089/fpd.2018.2559
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发表时间：
2019-04-09
影响因子：
2.8
通讯作者：
Xia, Xiaodong
中科院分区：
文献类型：
--
作者：
Fan, Qiuxia;Yan, Chunhong;Xia, Xiaodong
辅酶Q0 （CoQ0）是苯醌类化合物之一，已被证明具有抗肿瘤、抗炎和抗氧化活性。然而，其抗菌作用尚未得到广泛报道。本研究通过测定抑菌区、最低抑菌浓度（MIC）和生长曲线来评价CoQ0对金黄色葡萄球菌的抑菌活性。采用时间杀菌法测定了辅酶q0对色氨酸豆汤和巴氏杀菌奶中金黄色葡萄球菌的杀菌活性。通过测量细胞内ATP浓度、膜电位和细胞形态的变化来探索可能的作用机制。此外，采用碘化丙啶（PI）染色法评价CoQ0对细胞膜完整性的影响。CoQ0对受试菌株的MIC范围为7.8 ~ 62.5 μ g/mL。CoQ0在2倍MIC下对胰蛋白酶豆汤（TSB）和巴氏杀菌奶中的金黄色葡萄球菌有杀菌作用。CoQ0处理后，细胞内ATP浓度和膜电位下降。PI染色显示细菌细胞膜被破坏。通过场发射扫描电镜证实，CoQ0还会引起细胞形态的异常变化。这些发现表明，辅酶q0对金黄色葡萄球菌具有抗菌作用，部分原因是它具有破坏细胞膜的能力。
Coenzyme Q0 (CoQ0), one of benzoquinone compounds, has been demonstrated to possess antineoplastic, anti-inflammatory and antioxidant activities. However, its antimicrobial effect has not been extensively reported. In this study, antimicrobial activity of CoQ0 against Staphylococcus aureus was evaluated by measurement of inhibition zone, minimum inhibitory concentration (MIC), and growth curves. Time-kill assay was performed to assess the bactericidal activity of CoQ0 against S. aureus in tryptone soya broth and pasteurized milk. The possible mechanism of action was explored through measuring changes in intracellular ATP concentrations, membrane potential, and cell morphology. Furthermore, propidium iodide (PI) staining assay was performed to evaluate the effect of CoQ0 on cell membrane integrity. The MIC of CoQ0 against tested strains ranged from 7.8 to 62.5 mu g/mL. CoQ0 at 2 x MIC showed bactericidal effect on S. aureus in tryptic soy broth (TSB) and pasteurized milk. Decrease in intracellular ATP concentration and membrane potential were detected when cells were treated with CoQ0. PI staining demonstrated destruction of bacterial cell membrane. CoQ0 also induced abnormal cell morphological changes, as confirmed by field emission scanning electron microscopy. These findings suggested that CoQ0 exhibited antimicrobial effect on S. aureus, which was partly because of its ability to damage cell membrane.