DEVELOPMENTAL HISTORY AFFECTS THE SUSCEPTIBILITY OF SPINACH LEAVES TO INVIVO LOW-TEMPERATURE PHOTOINHIBITION
DEVELOPMENTAL HISTORY AFFECTS THE SUSCEPTIBILITY OF SPINACH LEAVES TO INVIVO LOW-TEMPERATURE PHOTOINHIBITION
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DOI：
10.1104/pp.99.3.1141
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发表时间：
1992-07-01
期刊：
影响因子：
7.4
通讯作者：
HUNER, NPA
中科院分区：
文献类型：
--
作者：
BOESE, SR;HUNER, NPA
室温叶绿素荧光被用来确定的影响，发展历史，发展阶段，和叶龄的敏感性菠菜在体内低温（5 ℃）诱导的光抑制。菠菜（菠菜品种萨沃伊）叶扩大在冷硬化温度（5 ℃的白天/晚上），250微摩尔每平方米每秒的光合质子通量密度的辐照度，和16小时的光周期是不太敏感比叶扩大在nonhardening温度（16或25 ℃的白天/晚上）和相同的辐照度和光周期。这种对低温光抑制的不同敏感性在高（1200）但不低（500或800微摩尔/平方米每秒）辐照处理下观察到。尽管对光抑制的敏感性不同，但冷硬化和非硬化菠菜在20或5 ℃时表现出相似的恢复动力学。将植物在16摄氏度（白天/夜晚）到5摄氏度（白天/夜晚）生长12天后，完全叶扩展没有改变在5摄氏度的光抑制的敏感性。相反，在5 ℃（白天/夜晚）至16 ℃（白天/夜晚）下生长12天的转移植物产生了与在16 ℃下生长的对照植物相似的在5 ℃下对光抑制的敏感性。因此，在5 ℃下生长期间获得的任何对低温光抑制的抗性在16 ℃下12天内丧失。我们的结论是，叶的发育历史，发育阶段，叶龄在体内菠菜的光抑制反应的贡献显着。因此，在研究植物对光抑制的敏感性时，必须明确这些特性。
Room temperature chlorophyll a fluorescence was used to determine the effects of developmental history, developmental stage, and leaf age on susceptibility of spinach to in vivo low temperature (5-degrees-C) induced photoinhibition. Spinach (Spinacia oleracea cv Savoy) leaves expanded at cold hardening temperatures (5-degrees-C day/night), an irradiance of 250 micromoles per square meter per second of photosynthetic proton flux density, and a photoperiod of 16 hours were less sensitive than leaves expanded at nonhardening temperatures (16 or 25-degrees-C day/night) and the same irradiance and photoperiod. This differential sensitivity to low-temperature photoinhibition was observed at high (1200) but not lower (500 or 800 micromoles per square meter per second) irradiance treatment. In spite of a differential sensitivity to photoinhibition, both cold-hardened and nonhardened spinach exhibited similar recovery kinetics at either 20 or 5-degrees-C. Shifting plants grown at 16-degrees-C (day/night) to 5-degrees-C (day/night) for 12 days after full leaf expansion did not alter the sensitivity to photoinhibition at 5-degrees-C. Conversely, shifting plants grown at 5-degrees-C (day/night) to 16-degrees-C (day/night) for 12 days produced a sensitivity to photoinhibition at 5-degrees-C similar to control plants grown at 16-degrees-C. Thus, any resistance to low-temperature photoinhibition acquired during growth at 5-degrees-C was lost in 12 days at 16-degrees-C. We conclude that leaf developmental history, developmental stage, and leaf age contribute significantly to the in vivo photoinhibitory response of spinach. Thus, these characteristics must be defined clearly in studies of plant susceptibility to photoinhibition.