FUNCTIONAL-ANALYSIS OF HUMAN FOAMY VIRUS ACCESSORY READING FRAMES
FUNCTIONAL-ANALYSIS OF HUMAN FOAMY VIRUS ACCESSORY READING FRAMES
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DOI：
10.1128/jvi.67.9.5411-5418.1993
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发表时间：
1993-09-01
影响因子：
5.4
通讯作者：
RETHWILM, A
中科院分区：
文献类型：
--
作者：
BAUNACH, G;MAURER, B;RETHWILM, A
泡沫病毒属于基因组结构复杂的逆转录病毒。人泡沫病毒（HFV）分离物在基因组的3'区具有三个开放阅读框（所谓的bel基因），据报道，其通过选择性剪接可能产生六种不同的蛋白质（W. Muranyi和R. M. Flugel，J. Virol. 65：727-735，1991）。为了分析HFV体外复制对这些蛋白的需求，我们构建了一组HFV感染性分子克隆的单个和组合bel基因突变体。发现缺乏反式激活因子bel-1的突变体不能复制。所有其他突变体复制得同样好，并产生相当滴度的感染性无细胞病毒。当HFV前病毒被置于异源启动子（猿猴病毒40）的控制下时，没有发现任何辅助基因产物是表达结构（gag）蛋白所需的。没有证据表明转录后调节蛋白存在于其他复杂的逆转录病毒中。
Foamy viruses belong to the retroviruses which possess a complex genome structure. The human foamy virus (HFV) isolate bears three open reading frames (the so-called bel genes) in the 3' region of the genome which have been reported to give rise to possibly six different proteins via alternative splicing (W. Muranyi and R. M. Flugel, J. Virol. 65:727-735, 1991). In order to analyze the requirements of these proteins for HFV replication in vitro, we constructed a set of single and combinatory bel gene mutants of an infectious molecular clone of HFV. The mutant which lacked the transacting activator, bel-1, was found to be replication incompetent. All other mutants replicated equally well and gave rise to comparable titers of infectious cell-free virus. When HFV proviruses were put under the control of a heterologous promoter (simian virus 40), none of the accessory gene products was found to be required for expression of structural (gag) proteins. There was no evidence for a posttranscriptional regulatory protein that is present in other complex retroviruses.