TISSUE-SPECIFIC AND DEVELOPMENTAL EXPRESSION OF RAT LONG-AND MEDIUM-CHAIN ACYL-COA DEHYDROGENASES
TISSUE-SPECIFIC AND DEVELOPMENTAL EXPRESSION OF RAT LONG-AND MEDIUM-CHAIN ACYL-COA DEHYDROGENASES
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DOI：
10.1016/0167-4781(93)90015-6
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发表时间：
1993-12-14
期刊：
影响因子：
--
通讯作者：
STRAUSS, AW
中科院分区：
文献类型：
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作者：
HAINLINE, BE;KAHLENBECK, DJ;STRAUSS, AW
由于线粒体β氧化酶表达的变化，哺乳动物组织和发育过程中脂肪酸的能量利用率各不相同。为了辨别两个相关的核基因是否表达相似，比较了大鼠长链和中链酰基辅酶A脱氢酶（LCAD和MCAD）mRNA的组织分布和发育概况。从新生大鼠主动脉的一个1451碱基的全长LCAD cDNA被用来研究mRNA在成年和胎鼠组织中的积累。LCAD和MCAD mRNA在主动脉、心脏和棕色脂肪中的表达水平是肝脏、肾脏和十二指肠的8-40倍。脑、胎盘、卵巢、睾丸和骨骼肌的mRNA表达量最少。用抗大鼠LCAD抗血清对成人组织进行Western印迹，显示相应量的LCAD蛋白亚基。LCAD mRNA在胎鼠心、肝、肾和脑中均有表达，并随胎龄增加而增加。从新生儿期到断奶期结束，LCAD和MCAD mRNA在棕色脂肪中的含量是其他组织的2-10倍。LCAD和MCAD mRNA在主动脉、心脏和棕色脂肪中的高水平表达可能反映了这些组织的高能量需求。LCAD和MCAD mRNA的差异表达不仅反映了内在的基因规定程序，还反映了外部影响，如激素和饮食。
Utilization of fatty acids for energy varies among mammalian tissues and during development due to changes in expression of enzymes of mitochondrial beta oxidation. To discern whether two related nuclear genes are expressed similarly, the tissue distribution and developmental profile of the rat long- and medium-chain acyl-CoA dehydrogenase (LCAD and MCAD) mRNAs were compared. A 1451 base full-length LCAD cDNA from neonatal rat aorta was used to study mRNA accumulation in adult and fetal rat tissues. LCAD and MCAD mRNAs were expressed in aorta, heart, and brown fat at levels 8-40 fold greater than in liver, kidney, and duodenum. Brain, placenta, ovary, testes, and skeletal muscle showed the least mRNA. Western blots of adult tissues with anti-rat LCAD antiserum showed corresponding amounts of LCAD protein subunits. LCAD mRNA was detectable in heart, liver, kidney, and brain of fetal rats and increased with age. LCAD and MCAD mRNAs were present in brown fat in 2-10 fold greater amounts compared to other tissues from the newborn period to the end of the weaning period. The high level of expression of LCAD and MCAD mRNA in aorta, heart, and brown fat likely reflects the high energy requirements of those tissues. Differential expression of LCAD and MCAD mRNAs reflects not only inherent gene prescribed programs, but also external influences such as hormones and diet.